Anti-KMT1B / SUV39H2抗体- C-terminal (ab71683)

概述

  • 产品名称
    Anti-KMT1B / SUV39H2抗体- C-terminal
    参阅全部 KMT1B / SUV39H2 一抗
  • 描述
    兔多克隆抗体to KMT1B / SUV39H2 - C-terminal
  • 经测试应用
    适用于: ICC/IF, ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human KMT1B/ SUV39H2 (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: Q9H5I1

  • 阳性对照
    • 293 and HL60 cell line lysates. Human breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab71683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/1000.
WB 1/250 - 1/500. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
IHC-P 1/50 - 1/100.

靶标

  • 功能
    Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher order chromatin organization during spermatogenesis.
  • 序列相似性
    Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 1 chromo domain.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • 结构域
    Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin.
  • 细胞定位
    Nucleus. Chromosome > centromere. Associates with centromeric constitutive heterochromatin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ23414 antibody
    • H3 K9 HMTase 2 antibody
    • H3-K9-HMTase 2 antibody
    • Histone H3 K9 methyltransferase 2 antibody
    • Histone H3-K9 methyltransferase 2 antibody
    • Histone lysine N methyltransferase H3 lysine 9 specific 2 antibody
    • Histone lysine N methyltransferase SUV39H2 antibody
    • Histone-lysine N-methyltransferase SUV39H2 antibody
    • KMT1B antibody
    • Lysine N methyltransferase 1B antibody
    • Lysine N-methyltransferase 1B antibody
    • sSuppressor of variegation 3 9 homolog 2 (Drosophila) antibody
    • Su(var)3 9 Drosophila homolog of 2 antibody
    • Su(var)3 9 homolog 2 antibody
    • Su(var)3-9 homolog 2 antibody
    • Suppressor of variegation 3 9 homolog 2 antibody
    • Suppressor of variegation 3-9 homolog 2 antibody
    • Suv39h2 antibody
    • SUV92_HUMAN antibody
    see all

图片

  • All lanes : Anti-KMT1B / SUV39H2 antibody - C-terminal (ab71683)

    Lane 1 : 293 cell line lysates
    Lane 2 : HL60 cell line lysates

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
  • Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ab71683 at 1/50 dilution. A peroxidase-conjugated secondary antibody was used, followed by DAB staining.
  • ICC/IF image of ab71683 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71683, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Mutonga M  et al. Targeting Suppressor of Variegation 3-9 Homologue 2 (SUV39H2) in Acute Lymphoblastic Leukemia (ALL). Transl Oncol 8:368-75 (2015). WB ; Human . Read more (PubMed: 26500027) »

See 1 Publication for this product

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