Anti-KMT1B / SUV39H2抗体(ab104343)

概述

  • 产品名称
    Anti-KMT1B / SUV39H2抗体
    参阅全部 KMT1B / SUV39H2 一抗
  • 描述
    兔多克隆抗体to KMT1B / SUV39H2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to Human KMT1B/ SUV39H2 aa 347-396 (internal sequence).
    Sequence:

    LDTRLPRIALFSTRTINAGEELTFDYQMKGSGDISSDSIDHSPAKKRVRT


    Database link: Q9H5I1

  • 阳性对照
    • HepG2 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab104343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher order chromatin organization during spermatogenesis.
  • 序列相似性
    Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 1 chromo domain.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • 结构域
    Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin.
  • 细胞定位
    Nucleus. Chromosome > centromere. Associates with centromeric constitutive heterochromatin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ23414 antibody
    • H3 K9 HMTase 2 antibody
    • H3-K9-HMTase 2 antibody
    • Histone H3 K9 methyltransferase 2 antibody
    • Histone H3-K9 methyltransferase 2 antibody
    • Histone lysine N methyltransferase H3 lysine 9 specific 2 antibody
    • Histone lysine N methyltransferase SUV39H2 antibody
    • Histone-lysine N-methyltransferase SUV39H2 antibody
    • KMT1B antibody
    • Lysine N methyltransferase 1B antibody
    • Lysine N-methyltransferase 1B antibody
    • sSuppressor of variegation 3 9 homolog 2 (Drosophila) antibody
    • Su(var)3 9 Drosophila homolog of 2 antibody
    • Su(var)3 9 homolog 2 antibody
    • Su(var)3-9 homolog 2 antibody
    • Suppressor of variegation 3 9 homolog 2 antibody
    • Suppressor of variegation 3-9 homolog 2 antibody
    • Suv39h2 antibody
    • SUV92_HUMAN antibody
    see all

图片

  • Anti-KMT1B / SUV39H2 antibody (ab104343) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 47 kDa

实验方案

文献

ab104343 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEF)
Permeabilization
Yes - 0.5% Triton x-100
Specification
MEF
Blocking step
BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Fixative
Formaldehyde
Username

Dr. Adam Burton

Verified customer

提交于 May 24 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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