概述

  • 产品名称Anti-KMT1A / SUV39H1抗体
    参阅全部 KMT1A / SUV39H1 一抗
  • 描述
    兔多克隆抗体to KMT1A / SUV39H1
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Fusion protein corresponding to Human KMT1A/ SUV39H1 (N terminal).
    Database link: 6839

  • 阳性对照
    • AOS-2 cell lysate, NIH/3T3 cell lysate and human breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab38637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
WB 1/100 - 1/500. Predicted molecular weight: 48 kDa.
ELISA 1/1000.
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation.
    • 序列相似性Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
      Contains 1 chromo domain.
      Contains 1 post-SET domain.
      Contains 1 pre-SET domain.
      Contains 1 SET domain.
    • 发展阶段Accumulates during mitosis at centromeres during prometaphase, but dissociates from the centromere at the meta- to anaphase transition.
    • 结构域Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin.
      In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.
    • 翻译后修饰Phosphorylated on serine residues, and to a lesser degree, on threonine residues. The phosphorylated form is stabilized by SBF1 and is less active in its transcriptional repressor function.
      Acetylated at Lys-266, leading to inhibition of enzyme activity. SIRT1-mediated deacetylation relieves this inhibition.
    • 细胞定位Nucleus. Nucleus lamina. Nucleus, nucleoplasm. Chromosome, centromere. Associates with centromeric constitutive heterochromatin.
    • Information by UniProt
    • 数据库链接
    • 别名
      • H3 K9 HMTase1 antibody
      • H3-K9-HMTase 1 antibody
      • Histone H3-K9 methyltransferase 1 antibody
      • Histone H3-K9 methyltransferase1 antibody
      • Histone lysine N methyltransferase, H3 lysine 9 specific 1 antibody
      • Histone-lysine N-methyltransferase SUV39H1 antibody
      • KMT1 A antibody
      • KMT1A antibody
      • Lysine N methyltransferase 1A antibody
      • Lysine N-methyltransferase 1A antibody
      • MG44 antibody
      • mIS6 antibody
      • Position-effect variegation 3-9 homolog antibody
      • Su(var)3 9 homolog 1 antibody
      • Su(var)3-9 homolog 1 antibody
      • Suppressor of variegation 3 9 homolog 1 (Drosophila) antibody
      • Suppressor of variegation 3-9 homolog 1 antibody
      • SUV39 H1 antibody
      • SUV39H antibody
      • SUV39H1 antibody
      • SUV91_HUMAN antibody
      see all

    Anti-KMT1A / SUV39H1 antibody 图像

    • All lanes : Anti-KMT1A / SUV39H1 antibody (ab38637) at 1/100 dilution

      Lane 1 : SAOS-2 cell lysate
      Lane 2 : NIH/3T3 cell lysate


      Predicted band size : 48 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
      Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
    • ab38637 at 1/50 staining human HEK293 cells by ICC/IF. The cells were formaldehyde fixed, blocked with BSA and incubated with the antibody for 1 hour. A goat anti-rabbit IgG was used as the secondary.

      See Abreview

    • ab38637 staining cultured human HeLa cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C.  The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C.  A Cy3® conjugated donkey anti-rabbit antibody diluted 1/400 was used as the secondary.

      See Abreview

    Anti-KMT1A / SUV39H1 antibody (ab38637)参考文献

    This product has been referenced in:
    • Alkemade FE  et al. Prenatal exposure to apoE deficiency and postnatal hypercholesterolemia are associated with altered cell-specific lysine methyltransferase and histone methylation patterns in the vasculature. Am J Pathol 176:542-8 (2010). IHC-P ; Mouse . Read more (PubMed: 20035052) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cultured Cells (HeLa cells)
    Specification HeLa cells
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton X100
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Alexander Rapp

    Verified customer

    提交于 Apr 18 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HEK293)
    Specification HEK293
    Fixative Formaldehyde
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10
    Username

    Abcam user community

    Verified customer

    提交于 Jan 15 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"