概述

  • 产品名称Anti-KMT1A / SUV39H1抗体
    参阅全部 KMT1A / SUV39H1 一抗
  • 描述
    兔多克隆抗体to KMT1A / SUV39H1
  • 特异性ab33056 recognises Histone-lysine N-methyltransferase, H3 lysine-9 specific 1(SUV39H1).
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: TFDYNMQVDP VDMESTRMDS NFGLAGLPGS PKKRVRIECK CGTESCRKYL, corresponding to C terminal amino acids 362-411 of Human KMT1A/ SUV39H1

  • 阳性对照
    • HepG2 cell lysate, human intestine.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab33056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation.
  • 序列相似性Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 1 chromo domain.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • 发展阶段Accumulates during mitosis at centromeres during prometaphase, but dissociates from the centromere at the meta- to anaphase transition.
  • 结构域Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin.
    In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.
  • 翻译后修饰Phosphorylated on serine residues, and to a lesser degree, on threonine residues. The phosphorylated form is stabilized by SBF1 and is less active in its transcriptional repressor function.
    Acetylated at Lys-266, leading to inhibition of enzyme activity. SIRT1-mediated deacetylation relieves this inhibition.
  • 细胞定位Nucleus. Nucleus lamina. Nucleus, nucleoplasm. Chromosome, centromere. Associates with centromeric constitutive heterochromatin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • H3 K9 HMTase1 antibody
    • H3-K9-HMTase 1 antibody
    • Histone H3-K9 methyltransferase 1 antibody
    • Histone H3-K9 methyltransferase1 antibody
    • Histone lysine N methyltransferase, H3 lysine 9 specific 1 antibody
    • Histone-lysine N-methyltransferase SUV39H1 antibody
    • KMT1 A antibody
    • KMT1A antibody
    • Lysine N methyltransferase 1A antibody
    • Lysine N-methyltransferase 1A antibody
    • MG44 antibody
    • mIS6 antibody
    • Position-effect variegation 3-9 homolog antibody
    • Su(var)3 9 homolog 1 antibody
    • Su(var)3-9 homolog 1 antibody
    • Suppressor of variegation 3 9 homolog 1 (Drosophila) antibody
    • Suppressor of variegation 3-9 homolog 1 antibody
    • SUV39 H1 antibody
    • SUV39H antibody
    • SUV39H1 antibody
    • SUV91_HUMAN antibody
    see all

Anti-KMT1A / SUV39H1 antibody 图像

  • Anti-KMT1A / SUV39H1 antibody (ab33056) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    gel concentration: 12%.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling KMT1A / SUV39H1 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of intestinal gland.

Anti-KMT1A / SUV39H1 antibody (ab33056)参考文献

This product has been referenced in:
  • Sidler C  et al. Immunosenescence is associated with altered gene expression and epigenetic regulation in primary and secondary immune organs. Front Genet 4:211 (2013). WB ; Rat . Read more (PubMed: 24151501) »

See 1 Publication for this product

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