特异性The monoclonal antibody 2F1 reacts with mouse killer cell lectin-like receptor G1 (KLRG1; formerly known as mouse MAFA or 2F1 Ag). This antibody stains 30-60% of NK1.1+ CD3- splenocytes, and a small fraction of T cells in all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.2µg for 106 cells.
Use at an assay dependent dilution.
功能Plays an inhibitory role on natural killer (NK) cells and T-cell functions upon binding to their non-MHC ligands. May mediate missing self recognition by binding to a highly conserved site on classical cadherins, enabling it to monitor expression of E-cadherin/CDH1, N-cadherin/CDH2 and R-cadherin/CDH4 on target cells.
组织特异性Expressed specifically on natural killer (NK) cells and T-cells, mainly CD8 T-cells.
序列相似性Contains 1 C-type lectin domain.
结构域Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. Upon phosphorylation of ITIM motif KLRG1 associates with the two phosphatases PTPN11 and INPP5D.
Jennings RN et al. Type I interferon signaling enhances CD8+ T cell effector function and differentiation during murine gammaherpesvirus 68 infection. J Virol88:14040-9 (2014).
Read more (PubMed: 25253356) »
Allie SR et al. Critical role for all-trans retinoic acid for optimal effector and effector memory CD8 T cell differentiation. J Immunol190:2178-87 (2013).
Read more (PubMed: 23338237) »