概述

  • 产品名称
  • 描述
    兔多克隆抗体to Klotho
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Human
  • 免疫原

    Synthetic peptide of 17 amino acids (Mouse) (C terminal).

性能

应用

Our Abpromise guarantee covers the use of ab18131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 116 kDa.
ELISA 1/10000 - 1/100000.

靶标

  • 功能
    May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1.
    The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.
  • 组织特异性
    Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney.
  • 疾病相关
    Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement.
  • 序列相似性
    Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.
  • 结构域
    Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 239, and the second one lacks the essential Glu active site residue at position 872.
  • 翻译后修饰
    N-glycosylated.
  • 细胞定位
    Secreted and Cell membrane. Isoform 1 shedding leads to a soluble peptide.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Kl antibody
    • KLOT_HUMAN antibody
    • Klotho peptide antibody

实验方案

文献

This product has been referenced in:
  • Sun H  et al. Overexpression of Klotho suppresses liver cancer progression and induces cell apoptosis by negatively regulating wnt/ß-catenin signaling pathway. World J Surg Oncol 13:307 (2015). WB . Read more (PubMed: 26499380) »
  • Labrie V  et al. Serine racemase is associated with schizophrenia susceptibility in humans and in a mouse model. Hum Mol Genet 18:3227-43 (2009). WB ; Mouse . Read more (PubMed: 19483194) »

See all 3 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Rat Tissue lysate - whole (Hippocampus)
Loading amount
30 µg
Specification
Hippocampus
Gel Running Conditions
Non-reduced Denaturing (14% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Gareth Bowen

Verified customer

提交于 Apr 25 2013

Thank you very much for your interest in ab18131.
To our knowledge, ab18131 has not been tested in Human. Therefore, I can offer a discount off a future purchase if you buy ab18131 now, test it in Human and submit feedback to us in the form of an ...

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Thank you for your inquiry and I apologize for the delay in this response. The C-terminal immunizing peptide used to generate ab18131 is based on the 1014 amino acid sequence of membrane-bound Klotho. This antibody should only recognize the membr...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (choroid plexus, kidney)
Specification
choroid plexus, kidney
Fixative
Paraformaldehyde
Antigen retrieval step
None
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Jan 27 2006

Thank you for your enquiry. The antibody orignates outside of Abcam and the originator of the antibody never reveals an immunogen peptide sequence. All I can provide is: "17aa peptide sequence near the C-terminus of mouse Klotho." I hope this inf...

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Thank you for your email. We do not carry klotho recombinant protein. I did a Google search, but unfortunately couldn't see any companies that do sell it. I'm still looking into your original question regarding ab18131 and will get back to you once...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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