Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 307-337 of Human KLHL9
Mouse testis tissue lysate and Human prostate carcinoma tissue.
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
ab104084 is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 69 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
KLHL9 is the substrate-specific adapter for a CUL3-based E3 ubiquitin-protein ligase complex. Within this complex, KLHL9 controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis.
Cul3-RING ubiquitin ligase complex; midbody
Kelch like 9 (Drosophila) antibody
Western blot - KLHL9 antibody (ab104084)
Anti-KLHL9 antibody (ab104084) at 1/100 dilution + Mouse testis tissue lysate at 35 µg
Predicted band size: 69 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KLHL9 antibody (ab104084)
ab104084, at 1/50 staining KLHL9 by immunohistochemistry in formalin fixed and paraffin embedded Human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"