Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 307-337 of Human KLHL9
Mouse testis tissue lysate and Human prostate carcinoma tissue.
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
ab104084 is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 69 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
KLHL9 is the substrate-specific adapter for a CUL3-based E3 ubiquitin-protein ligase complex. Within this complex, KLHL9 controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis.
Cul3-RING ubiquitin ligase complex; midbody
Kelch like 9 (Drosophila) antibody
Anti-KLHL9 antibody 图像
Western blot - KLHL9 antibody (ab104084)
Anti-KLHL9 antibody (ab104084) at 1/100 dilution + Mouse testis tissue lysate at 35 µg
Predicted band size : 69 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KLHL9 antibody (ab104084)
ab104084, at 1/50 staining KLHL9 by immunohistochemistry in formalin fixed and paraffin embedded Human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Anti-KLHL9 antibody (ab104084)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"