The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 84 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
相关性The kelch repeat protein family is an actin binding protein. It is characterized by tandemly arranged motifs of about 50 amino acids. Most members of the kelch repeat family were cytoskeletal proteins implicated in various cellular processes, such as actin cytoskeleton interaction, cytoplasmic sequestration of transcription factors and cell morphology. KLHL5 has high identity with Drosophila kelch protein and many other family members. A novel splicing variant of KLHL5, named KLHL5b, has been identified.