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A region within synthetic peptide: TPRGWHCAVT LSDRVYVMGG SQLGPRGERV DVLTVECYSP ATGQWSYAAP, corresponding to amino acids 501-550 of Human KLHL31
Our Abpromise guarantee covers the use of ab62181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling KLHL31 with ab62181 at 4-8µg/ml. Arrows indicate positively labelled skeletal muscle cells. Magnification: 400X.
ab62181 has not yet been referenced specifically in any publications.