概述

  • 产品名称
  • 描述
    兔多克隆抗体to KLHL3
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: EGESVKLSSQ TLIQAGDDEK NQRTITVNPA HMGKAFKVMN ELRSKQLLCD , corresponding to amino acids 2-51 of Human KLHL3

  • 阳性对照
    • HepG2 cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab66655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 65 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性
    KLHL3 protein contains a poxvirus and zinc finger domain at the N-terminus and six tandem repeats (kelch repeats) at the C-terminus. At the amino acid level, KLHL3 shares 77% similarity with Drosophila kelch and 89% similarity with Mayven (KLHL2), another human kelch homolog. At least three isoforms are produced and may be the result of alternative promoter usage. The KLHL3 maps within the smallest commonly deleted segment in myeloid leukemias characterized by a deletion of 5q; however, no inactivating mutations of KLHL3 could be detected in malignant myeloid disorders with loss of 5q.
  • 细胞定位
    Cytoplasmic and cytoskeleton.
  • 数据库链接
  • 别名
    • FLJ40871 antibody
    • kelch (Drosophila) like 3 antibody
    • kelch like 3 (Drosophila) antibody
    • kelch like 3 antibody
    • Kelch like protein 3 antibody
    • KIAA1129 antibody
    • KLHL 3 antibody
    • KLHL3 antibody
    • MGC44594 antibody
    see all

图片

  • Anti-KLHL3 antibody (ab66655) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Observed band size : >70 kDa (why is the actual band size different from the predicted?)

实验方案

文献

This product has been referenced in:
  • Roy A  et al. Generation of WNK1 knockout cell lines by CRISPR/Cas-mediated genome editing. Am J Physiol Renal Physiol 308:F366-76 (2015). Read more (PubMed: 25477473) »
  • Gong Y  et al. KLHL3 regulates paracellular chloride transport in the kidney by ubiquitination of claudin-8. Proc Natl Acad Sci U S A 112:4340-5 (2015). Read more (PubMed: 25831548) »

See all 3 Publications for this product

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