参阅全部 KLHL25 一抗
WB, ELISA, IHC-Pmore details
Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Pig
A region within synthetic peptide: QSEDFNSLSK DTLLDLISSD ELETEDERVV FEAILQWVKH DLEPRKVHLP, corresponding to N terminal amino acids 181-230 of Human KLHL25
- Jurkat cell lysate, human brain tissue.
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- 存储溶液Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
- 纯度Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 0.3125 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA titre using peptide based assay:1:1562500.
||Use a concentration of 4 - 8 µg/ml.
- 序列相似性Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats.
Information by UniProt
- 2810402K13Rik antibody
- BC027373 antibody
- Ectoderm neural cortex protein 2 antibody
Anti-KLHL25 antibody 图像
Western blot - KLHL25 antibody (ab55953)
Anti-KLHL25 antibody (ab55953) at 0.3125 µg/ml + Jurkat cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/100,000 dilution.
Predicted band size : 66 kDa
Observed band size : 66 kDa
Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL25 antibody (ab55953)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling KLHL25 with ab55953 at 4-8µg/ml Arrows indicate postively labelled neural cells. Magnification: 400X.
Anti-KLHL25 antibody (ab55953)参考文献
has not yet been referenced specifically in any publications.
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