Mouse, Rat, Cow
Recombinant fragment corresponding to a region within amino acids 345-520 of Human KLHL21
WB: A549, HeLa, Jurkat and Raji whole cell lysates
IHC-P: Hepatoma tissue
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 1.21% Tris, 20% Glycerol, 0.75% Glycine
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 67 kDa.
1/100 - 1/500. Perform heat mediated antigen retrieval with 10mM citrate buffer pH 6.0 or Tris-EDTA buffer pH 8.0 before commening with IHC staining protocol.
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for efficient chromosome alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
Protein modification; protein ubiquitination.
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain. Contains 6 Kelch repeats.
Cytoplasm > cytoskeleton > spindle. Localizes to the spindle midzone and targets CUL3 to this region.
Information by UniProt
Kelch-like protein 21 antibody
Western blot - Anti-KLHL21 antibody (ab111604)
Anti-KLHL21 antibody (ab111604) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 67 kDa 7.5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL21 antibody (ab111604)
ab111604, at a 1/500, staining KLHL21 in paraffin-embedded Hepatoma tissue.
has not yet been referenced specifically in any publications.
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