Anti-KLHL21抗体(ab111604)
Key features and details
- Rabbit polyclonal to KLHL21
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-KLHL21抗体 -
描述
兔多克隆抗体to KLHL21 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Recombinant fragment corresponding to a region within amino acids 345-520 of Human KLHL21
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阳性对照
- WB: A549, HeLa, Jurkat and Raji whole cell lysates IHC-P: Hepatoma tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab111604于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 67 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with 10mM citrate buffer pH 6.0 or Tris-EDTA buffer pH 8.0 before commening with IHC staining protocol.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 67 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with 10mM citrate buffer pH 6.0 or Tris-EDTA buffer pH 8.0 before commening with IHC staining protocol. |
靶标
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功能
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for efficient chromosome alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats. -
细胞定位
Cytoplasm > cytoskeleton > spindle. Localizes to the spindle midzone and targets CUL3 to this region. - Information by UniProt
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数据库链接
- Entrez Gene: 506632 Cow
- Entrez Gene: 9903 Human
- Entrez Gene: 242785 Mouse
- Entrez Gene: 313743 Rat
- SwissProt: Q08DS0 Cow
- SwissProt: Q9UJP4 Human
- SwissProt: Q3U410 Mouse
- SwissProt: D4A2K4 Rat
see all -
别名
- Kelch-like protein 21 antibody
- KIAA0469 antibody
- KLH21_HUMAN antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab111604 被引用在 2 文献中.
- Wang J et al. EZH2 Regulates the Correlation between Skin Regeneration and the Duration of Mechanical Stretch. J Invest Dermatol 141:894-902.e9 (2021). PubMed: 33069730
- Mei ZZ et al. Kelch-like Protein 21 (KLHL21) Targets I?B Kinase-ß to Regulate Nuclear Factor ?-Light Chain Enhancer of Activated B Cells (NF-?B) Signaling Negatively. J Biol Chem 291:18176-89 (2016). PubMed: 27387502