Anti-KLHL20抗体(ab67682)

概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to KLHL20
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length KLHL20 protein (Human)

  • 阳性对照
    • KLHL20 transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab67682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 68 kDa).

靶标

  • 功能
    Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis. Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 6 Kelch repeats.
  • 细胞定位
    Cytoplasm > perinuclear region. Nucleus. Localizes in the perinuclear region in normal conditions. Following IFN-alpha or IFN-gamma treatment, it is relocalized and sequestrated to the PML nuclear bodies, preventing DAPK1 ubiquitination.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Kelch like ECT2 interacting protein antibody
    • Kelch like protein 20 antibody
    • Kelch like protein X antibody
    • Kelch motif containing protein antibody
    • Kelch-like ECT2-interacting protein antibody
    • Kelch-like protein 20 antibody
    • Kelch-like protein X antibody
    • KHLHX antibody
    • KLEIP antibody
    • KLH20_HUMAN antibody
    • KLHL20 antibody
    • KLHLX antibody
    • RP3-383J4.3 antibody
    see all

图片

  • All lanes : Anti-KLHL20 antibody (ab67682) at 1/500 dilution

    Lane 1 : KLHL20 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 68 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab67682 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
Loading amount
90 µg
Specification
Human osteosarcoma U2OS cells
Gel Running Conditions
Reduced Denaturing (4-18% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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提交于 Apr 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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