Anti-KLHL20抗体(ab108238)

概述

  • 产品名称Anti-KLHL20抗体
    参阅全部 KLHL20 一抗
  • 描述
    兔多克隆抗体to KLHL20
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within C-terminal amino acids 481-530 (

    NPQENRWHTIAPMGTRRKHLGCAVYQDMIYAVGGRDDTTELSSAERYNPR

    ) of Human KLHL20 (NP_055273).

  • 阳性对照
    • HeLa cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab108238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis. Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 6 Kelch repeats.
  • 细胞定位Cytoplasm > perinuclear region. Nucleus. Localizes in the perinuclear region in normal conditions. Following IFN-alpha or IFN-gamma treatment, it is relocalized and sequestrated to the PML nuclear bodies, preventing DAPK1 ubiquitination.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Kelch like ECT2 interacting protein antibody
    • Kelch like protein 20 antibody
    • Kelch like protein X antibody
    • Kelch motif containing protein antibody
    • Kelch-like ECT2-interacting protein antibody
    • Kelch-like protein 20 antibody
    • Kelch-like protein X antibody
    • KHLHX antibody
    • KLEIP antibody
    • KLH20_HUMAN antibody
    • KLHL20 antibody
    • KLHLX antibody
    • RP3-383J4.3 antibody
    see all

Anti-KLHL20 antibody 图像



  • Predicted band size : 68 kDa
    Western blot analysis of MCF7 cell lysate labeling KLHL20 with ab108238 at 1.0µg/ml.
  • Anti-KLHL20 antibody (ab108238) at 1 µg/ml + HeLa Cell Lysate at 10 µg

    Predicted band size : 68 kDa
    12% SDS PAGE

Anti-KLHL20 antibody (ab108238)参考文献

ab108238 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
Loading amount 90 µg
Specification Human osteosarcoma U2OS cells
Gel Running Conditions Reduced Denaturing (4-18% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Apr 11 2012

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