antigen sequence: AGAWESVAPM NIRRSTHDLV AMDGWLYAVG GNDGSSSLNS IEKYNPRTNK WVAASCMFTR RSSVGVAVLE LLNFPPPSSP TLSVS, corresponding to amino acids 554-638 of Human KLHL17.
Human testis tissue; RT 4, U 51 MG and Human plasma lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Substrate-recognition component of some cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex. The BCR(KLHL17) mediates the ubiquitination and subsequenct degradation of GLUR6. May play a role in the actin-based neuronal function.
Protein modification; protein ubiquitination.
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain. Contains 6 Kelch repeats.
Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Postsynaptic density.
Information by UniProt
Immunocytochemistry/ Immunofluorescence - Anti-KLHL17 antibody (ab122164)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122164 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL17 antibody (ab122164)
ab122164 at 1/100 dilution staining KLHL17 in Paraffin-embedded Human testis tissue by Immunohistochemistry.
Western blot - Anti-KLHL17 antibody (ab122164)
All lanes : Anti-KLHL17 antibody (ab122164) at 1/250 dilution Lane 1 : RT 4 lysate Lane 2 : U 251 MG lysate Lane 3 : Human plasma lysate Lane 4 : Human liver lysate Lane 5 : Human tonsil lysate Developed using the ECL technique
has not yet been referenced specifically in any publications.
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