Anti-KLF9抗体(ab26074)

概述

  • 产品名称Anti-KLF9抗体
    参阅全部 KLF9 一抗
  • 描述
    兔多克隆抗体to KLF9
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Pig
  • 免疫原

    Synthetic peptide derived from a region between residues 36-86 of human KLF9.

    .

  • 阳性对照
    • Jurkat cell lysate and Human kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab26074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

Anti-KLF9 antibody 图像

  • Anti-KLF9 antibody (ab26074) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + Jurkat whole cell lysate (ab7899)

    Secondary
    HRP conjugated anti-Rabbit IgG

    Predicted band size : 27 kDa
    Observed band size : 27,42 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical detection of KLF9 using 4 ug/ml ab26074 in paraffin embedded human kidney tissue section. Arrows indicate positively labelled epithelial cells of the renal tubule.
  • ICC/IF image of ab26074 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26074, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-KLF9 antibody (ab26074)参考文献

ab26074 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I matched up the sequence for the peptide that this antibody is against for human and mouse and they were 100% homologous. I hope this information helps, please do not hesitate to contact us if you need any more advice or...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"