The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明Western Blot: Approx 50kDa band seen in Human Kidney lysate.
Predicted molecular weight: 40 kDa.
Recommended for use at 1.0-3.0µg/ml.
功能Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK).
序列相似性Belongs to the Sp1 C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers.
翻译后修饰Sumoylation at Lys-67 represses transcriptional activity and reduces cell cycle progression into the G(1) phase. Has no effect on subcellular location.