Anti-KLF5抗体(ab24331)
Key features and details
- Rabbit polyclonal to KLF5
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-KLF5抗体
参阅全部 KLF5 一抗 -
描述
兔多克隆抗体to KLF5 -
宿主
Rabbit -
特异性
Some of our customers have successfully used ab24331 on mouse, please see PubMed ID: 19720868 and 21600198. However, we have been unable to show cross-reactivity with this species and therefore we cannot guarantee this product will consistently work on mouse. Please contact our Scientific Support team for further information. -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab24331于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 0.6 - 3 µg/ml.
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WB | (1) |
Use a concentration of 2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).
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说明 |
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IHC-P
Use a concentration of 0.6 - 3 µg/ml. |
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa). |
靶标
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功能
Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes. -
组织特异性
Expressed only in testis and placenta. -
序列相似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
翻译后修饰
Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 688 Human
- Entrez Gene: 84410 Rat
- Omim: 602903 Human
- SwissProt: Q13887 Human
- Unigene: 508234 Human
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形式
KLF5 (also known as basic transcription element-binding protein 2, BTEB2, and intestine enriched krüppel-like factor, IKLF), appears to play a central role in the regulation of intestinal epithelial renewal in both the small intestine and colon. KLF5 also regulates adipocyte differentiation. -
别名
- Basic transcription element binding protein 2 antibody
- Basic transcription element-binding protein 2 antibody
- BTE binding protein 2 antibody
see all
图片
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Anti-KLF5 antibody (ab24331) at 2 µg/ml + Small Intestine (Human) Tissue Lysate at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
The image shows nuclear staining of the epithelial cells of the intestinal gland. Tris EDTA pH9 and Citrate pH6 are both suitable antigen retrieval techniques.
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All lanes : Anti-KLF5 antibody (ab24331) at 1/500 dilution
Lane 1 : Marker ladder
Lane 2 : Cell lysate prepared from human Ls174T cells at 40 µg
Lane 3 : Cell lysate prepared from IEC-6 rat intestinal cells at 40 µg
Secondary
Lanes 2-3 : HRP conjugated goat polyclonal to rabbit IgG at 1/1500 dilution
Predicted band size: 50 kDa
Exposure time: 2 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (35)
ab24331 被引用在 35 文献中.
- Schmidt A et al. Deciphering Pro-angiogenic Transcription Factor Profiles in Hypoxic Human Endothelial Cells by Combined Bioinformatics and in vitro Modeling. Front Cardiovasc Med 9:877450 (2022). PubMed: 35783871
- Chen W et al. KLF5 Is Activated by Gene Amplification in Gastric Cancer and Is Essential for Gastric Cell Proliferation. Cells 10:N/A (2021). PubMed: 33923166
- Sun Q et al. Effect of lncRNA PVT1/miR186/KLF5 Axis on the Occurrence and Progression of Cholangiocarcinoma. Biomed Res Int 2021:8893652 (2021). PubMed: 34337058
- Takagi Y et al. High expression of Krüppel-like factor 5 is associated with poor prognosis in patients with colorectal cancer. Cancer Sci 111:2078-2092 (2020). PubMed: 32279400
- Ma JB et al. KLF5 inhibits STAT3 activity and tumor metastasis in prostate cancer by suppressing IGF1 transcription cooperatively with HDAC1. Cell Death Dis 11:466 (2020). PubMed: 32546700