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Our Abpromise guarantee covers the use of ab72543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). We recommend blocking with 5% nonfat dry milk in TBST for 30-60 minutes. Incubate the membrane overnight with the primary antibody in 1% milk in TBST.|
|IHC-P||Use a concentration of 10 µg/ml.|
|Flow Cyt||Use 1µg for 101-7 cells.
This antibody has been tested in an intracellular protocol.