概述

  • 产品名称
  • 描述
    兔多克隆抗体to KLF4
  • 特异性
    ab106629 will detect all three known isoforms. It does not cross-react with other KLF members.
  • 经测试应用
    适用于: ChIP, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 20 amino acid peptide from near the C terminal of Human KLF4 (AAH30811).

  • 阳性对照
    • Human liver tissue lysate; Rat liver tissue.

应用

Our Abpromise guarantee covers the use of ab106629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration. PubMed: 25644173
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.

靶标

  • 功能
    Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
  • 序列相似性
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • 结构域
    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Endothelial Kruppel like zinc finger protein antibody
    • Epithelial zinc finger protein EZF antibody
    • EZF antibody
    • GKLF antibody
    • gut Kruppel-like factor antibody
    • Gut-enriched krueppel-like factor antibody
    • KLF antibody
    • KLF4 antibody
    • KLF4_HUMAN antibody
    • Krueppel-like factor 4 antibody
    • Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor) antibody
    • Kruppel like factor 4 (gut) antibody
    see all

图片

  • Lane 1 : Anti-KLF4 antibody (ab106629) at 1 µg/ml
    Lane 2 : Anti-KLF4 antibody (ab106629) at 2 µg/ml

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human liver tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 55 kDa
  • ab106629 at 5 ug/ml, staining KLF4 in Rat liver by Immunohistochemistry, Paraffin-embedded tissue.

文献

This product has been referenced in:
  • Dubuissez M  et al. Protein Kinase C-Mediated Phosphorylation of BCL11B at Serine 2 Negatively Regulates Its Interaction with NuRD Complexes during CD4+ T-Cell Activation. Mol Cell Biol 36:1881-98 (2016). Read more (PubMed: 27161321) »
  • Li W  et al. Genome-wide analyses identify KLF4 as an important negative regulator in T-cell acute lymphoblastic leukemia through directly inhibiting T-cell associated genes. Mol Cancer 14:26 (2015). ChIP ; Mouse . Read more (PubMed: 25644173) »

See all 2 Publications for this product

客户评价及客户问答

Abreviews
Application
CyTOF
Sample
Mouse Cell (Lung)
Specification
Lung
Username

Irit Milman

Verified customer

提交于 Nov 14 2017

Thank you for your participation in Abcam's follow up survey.

I have checked for alternative antibodies that were tested in WB as well as with mouse:

ab106629:http://www.abcam.com/klf4-antibody-ab106629.html

We also have a...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册