概述

  • 产品名称
  • 描述
    兔多克隆抗体to KLF3
  • 经测试应用
    适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human KLF3 aa 53-102 (N terminal).
    Sequence:

    LSHGIQMEPVDLTVNKRSSPPSAGNSPSSLKFPSSHRRASPGLSMPSSSP

  • 阳性对照
    • HepG2 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab49221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 23091163
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution.

靶标

图片

  • Anti-KLF3 antibody (ab49221) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

文献

This product has been referenced in:
  • Shao M  et al. Characterization and phylogenetic analysis of Krüppel-like transcription factor (KLF) gene family in tree shrews (Tupaia belangeri chinensis). Oncotarget 8:16325-16339 (2017). Read more (PubMed: 28032601) »
  • Seifert M  et al. Cellular origin and pathophysiology of chronic lymphocytic leukemia. J Exp Med 209:2183-98 (2012). ICC/IF ; Human . Read more (PubMed: 23091163) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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