概述

  • 产品名称
  • 描述
    山羊多克隆抗体to KLF15
  • 宿主
    Goat
  • 经测试应用
    适用于: IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Pig
  • 免疫原

    Synthetic peptide: VDHLLPVDENFSS, corresponding to N terminal amino acids 2-14 of Human KLF15.

  • 阳性对照
    • Human Liver or HepG2 lysate
  • 常规说明

    Principal Names – KLF15; Kruppel-like factor 15; KKLF; KKLF protein; kidney-enriched Kruppel-like factor Official Gene Symbol - KLF15 GenBank Accession Number – NP_054798 LocusLink ID - 28999 (human); 66277 (mouse); 85497 (rat)

     

    We no longer guarantee this product for mouse because of recent complaints. The immunogen however shares 92% sequence homology with mouse so we still predict the antibody will cross-react. We will welcome any feedback from customers.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration. PubMed: 24407292
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).Can be blocked with Human KLF15 peptide (ab22927).

Approx 48kDa band observed in lysates of hepatoblastoma cell line HepG2 (calculated MW of 44.0kDa according to NP_054798.1). Older batches detected a band of approximately 65 kDa in Human Liver lysate(predicted molecular weight: 46 kDa). Note the higher than expected size is in agreement with previous Western results (Mol Cell Biol 2000 Oct;20(19):7319-31).

IHC-P Use at an assay dependent concentration. PubMed: 26378916

靶标

图片

  • ab2647 staining (2µg/ml) of Human Liver lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 12 hours.  Detected by western blot using chemiluminescence. ab2647 staining (1 µg/ml) of Mouse skeletal muscle lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.
  • The level of KLF15 in human endometrium without (b) and with (d) endometriosis were performed by immunohistochemical analysis. Paraffin-embedded tissues were cut at 5 μm and mounted on silane-coated slides, deparaffinized, and rehydrated in a graded alcohol series before blocking with 10% normal goat serum in PBS (pH 7.5) and incubating with ab2647 diluted 1:5000 in 10% normal goat serum in PBS (pH 7.5) overnight at 4°C

  • Lane 1 : Anti-KLF15 antibody (ab2647) at 1 µg/ml
    Lane 2 : Anti-KLF15 antibody (ab2647) at 0.5 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 44 kDa

文献

This product has been referenced in:
  • Liang N  et al. Immunosuppressive effects of hydroxychloroquine and artemisinin combination therapy via the nuclear factor-?B signaling pathway in lupus nephritis mice. Exp Ther Med 15:2436-2442 (2018). WB ; Mouse . Read more (PubMed: 29456648) »
  • Monsivais D  et al. Uterine ALK3 is essential during the window of implantation. Proc Natl Acad Sci U S A 113:E387-95 (2016). IHC-P ; Mouse . Read more (PubMed: 26721398) »

See all 10 Publications for this product

客户评价及客户问答

There are currently no Customer reviews or Questions for ab2647.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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