Anti-KLF15抗体(ab22851)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to KLF15
  • 特异性
    ab22851 reacts with Krueppel-like factor 15 (KLF15).
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 29-83 of Human KLF15

  • 阳性对照
    • HepG2 cell lysate and human liver.

性能

应用

Our Abpromise guarantee covers the use of ab22851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Transcriptional activator. Binds to the GA element of the CLCNKA promoter.
  • 组织特异性
    Highly expressed in liver, followed by heart, skeletal muscle, and kidney. Not expressed in bone marrow or lymphoid tissues.
  • 序列相似性
    Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Kidney enriched Kruppel like factor antibody
    • Kidney-enriched krueppel-like factor antibody
    • KKLF antibody
    • KKLF protein antibody
    • KLF 15 antibody
    • KLF15 antibody
    • KLF15_HUMAN antibody
    • Krueppel-like factor 15 antibody
    • Kruppel like factor 15 antibody
    see all

Anti-KLF15 antibody 图像

  • Anti-KLF15 antibody (ab22851) at 4 µg/ml + HepG2 cell lysate.

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling KLF15 with ab22851 at a concentration of 4-8µg/ml. Positive stained hepatocytes marked by arrows. Magnification X400.

Anti-KLF15 antibody (ab22851)参考文献

ab22851 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Sheep Tissue lysate - whole (skeletal muscle tissue)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
50 µg
Specification
skeletal muscle tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Sep 29 2017

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