概述

  • 产品名称Anti-KLF10抗体
    参阅全部 KLF10 一抗
  • 描述
    兔多克隆抗体to KLF10
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Non Human Primates
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human KLF10.

    (Peptide available as ab73859.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Jurkat Whole Cell; Ramos Whole Cell; Human Skeletal Muscle Tissue; Human Brain Tissue; Mouse Liver Tissue; Mouse Testis Tissue and Rat Thymus Tissue.

性能

应用

Our Abpromise guarantee covers the use of ab73537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

靶标

  • 功能Transcriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. Plays a role in the regulation of the circadian clock; binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis (By similarity). May play a role in the cell cycle regulation.
  • 序列相似性Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • 翻译后修饰Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Early growth response, alpha antibody
    • Early growth response, alpha like antibody
    • EGR A antibody
    • EGR alpha antibody
    • EGR(A) antibody
    • EGR-alpha antibody
    • EGRA antibody
    • Egral antibody
    • EGRalpha antibody
    • Gdnfif antibody
    • KLF 10 antibody
    • KLF10 antibody
    • KLF10_HUMAN antibody
    • Krueppel like factor 10 antibody
    • Krueppel like factor10 antibody
    • Krueppel-like factor 10 antibody
    • Kruppel like factor 10 antibody
    • mGIF antibody
    • TGF-beta- inducible early gene antibody
    • TGFB inducible early growth response 1 antibody
    • TGFB inducible early growth response antibody
    • TGFB inducible early growth response protein 1 antibody
    • TGFB-inducible early growth response protein 1 antibody
    • TIEG 1 antibody
    • TIEG antibody
    • TIEG-1 antibody
    • TIEG1 antibody
    • Transforming growth factor beta inducible early growth response antibody
    • Transforming growth factor beta inducible early growth response protein 1 antibody
    • Transforming growth factor-beta-inducible early growth response protein 1 antibody
    • Zinc finger transcription factor TIEG antibody
    see all

Anti-KLF10 antibody 图像

  • All lanes : Anti-KLF10 antibody (ab73537) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 4 : Testis (Mouse) Tissue Lysate
    Lane 5 : Thymus (Rat) Tissue Lysate
    Lane 6 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 7 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

Anti-KLF10 antibody (ab73537)参考文献

This product has been referenced in:
  • Hung MS  et al. Knockdown of cullin 4A inhibits growth and increases chemosensitivity in lung cancer cells. J Cell Mol Med 20:1295-306 (2016). WB ; Human . Read more (PubMed: 26969027) »
  • Peng DJ  et al. Noncanonical K27-linked polyubiquitination of TIEG1 regulates Foxp3 expression and tumor growth. J Immunol 186:5638-47 (2011). WB ; Human . Read more (PubMed: 21471442) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Permeabilization No
Specification skin
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 22°C
Fixative Formaldehyde
Username

Miss. MC Branchet

Verified customer

提交于 Oct 07 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Breast carcinoma)
Specification Breast carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Dec 16 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"