This antibody gave a positive signal in the following lysates:
Jurkat Whole Cell; Ramos Whole Cell; Human Skeletal Muscle Tissue; Human Brain Tissue; Mouse Liver Tissue; Mouse Testis Tissue and Rat Thymus Tissue.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
功能Transcriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. Plays a role in the regulation of the circadian clock; binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis (By similarity). May play a role in the cell cycle regulation.
序列相似性Belongs to the Sp1 C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers.
翻译后修饰Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
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Anti-KLF10 antibody 图像
Western blot - KLF10 antibody (ab73537)
All lanes : Anti-KLF10 antibody (ab73537) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : Liver (Mouse) Tissue Lysate Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330) Lane 4 : Testis (Mouse) Tissue Lysate Lane 5 : Thymus (Rat) Tissue Lysate Lane 6 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 7 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 53 kDa Observed band size : 53 kDa Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 12 minutes
Anti-KLF10 antibody (ab73537)参考文献
This product has been referenced in:
Hung MS et al. Knockdown of cullin 4A inhibits growth and increases chemosensitivity in lung cancer cells. J Cell Mol Med20:1295-306 (2016).
Read more (PubMed: 26969027) »
Peng DJ et al. Noncanonical K27-linked polyubiquitination of TIEG1 regulates Foxp3 expression and tumor growth. J Immunol186:5638-47 (2011).
Read more (PubMed: 21471442) »