Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.
Belongs to the kinesin light chain family. Contains 6 TPR repeats.
All lanes : Anti-KLC2 antibody (ab95881) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 3 minutes
Immunoprecipitation - KLC2 antibody (ab95881)
ab95881 at 0.4 µg/ml detecting KLC2 in HeLa whole cell lysate by WB following IP.
Lane 1: KLC2 with 3 µg/mg of lysate
Lane 2: IP with an antibody which recognizes a downstream epitope of KLC2
Lane 3: control IgG
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Carpentier DC et al. Vaccinia virus protein complex F12/E2 interacts with kinesin light chain isoform 2 to engage the kinesin-1 motor complex. PLoS Pathog11:e1004723 (2015).
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