概述

  • 产品名称Anti-KLC1抗体[L2]
    参阅全部 KLC1 一抗
  • 描述
    小鼠单克隆抗体[L2] to KLC1
  • 特异性Reacts with the light chain of kinesin near the C-terminal.
  • 经测试应用适用于: WB, IP, ICC, RIAmore details
    不适用于: ICC/IF or IHC
  • 种属反应性
    与反应: Rat
  • 免疫原

    Full length protein (Cow). Bovine brain kinesin

  • 阳性对照
    • mouse brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab23586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 65-70 kDa (predicted molecular weight: 61 kDa). Do not dilute in buffers containing any other protein or detergent. See reference Lazarov O et al reference.
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
RIA Use at an assay dependent dilution.
  • 应用说明Is unsuitable for ICC/IF or IHC.
  • 靶标

    • 功能Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.
    • 组织特异性Found in a variety of tissues. Mostly abundant in brain and spine.
    • 序列相似性Belongs to the kinesin light chain family.
      Contains 6 TPR repeats.
    • 翻译后修饰Isoform I is phosphorylated on Ser-600. Isoform J is phosphorylated on Ser-631.
    • 细胞定位Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Kinesin 2 60/70kDa antibody
      • Kinesin light chain 1 antibody
      • Kinesin2 antibody
      • KLC 1 antibody
      • KLC antibody
      • Klc1 antibody
      • KLC1_HUMAN antibody
      • KNS 2 antibody
      • KNS 2A antibody
      • KNS2 antibody
      • KNS2A antibody
      • Medulloblastoma antigen MU MB 2.50 antibody
      • MGC15245 antibody
      see all

    Anti-KLC1 antibody [L2] (ab23586)参考文献

    This product has been referenced in:

    See all 5 Publications for this product

    Product Wall

    I'm delighted to be able to confirm that the correct band size for Kinesin Light Chain is actually 65-70kDa and that you are therefore seeing the correct band now. I apologize for the error on the datasheet, this has been corrected immediately. I h...

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    Thank you for your reply. I would recommend to incubate the membrane in 5%BSA (in TBST Tween20 0.1%) for 30min-1hour, then rinsing lightly (5 seconds) in TBST, and incubating the primary in TBST only.This should block well but not excessively. I hope t...

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    I have been informed today by the source of ab23586 that the positive control recommended for this antibody is brain lysate, as this tissue has the highest levels. The antibody should not be diluted in buffers containing any other protein or detergent...

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    Thank you for your reply and expressing your concern regarding a possible delay in the shipping of the vial. This could indeed be a possible reason for a damaged vial. However the only order in the US for this vial, to Yale, was order 115334 and this w...

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    I'm very sorry to hear you are experiencing a problem with ab23586. I am trying to find out from the source of this antibody what the recommended positive control is as I am concerned that your samples may not have high levels of kinesin light chai...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"