重组Anti-KLC1抗体[EPR12441(B)] (ab174273)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12441(B)] to KLC1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-KLC1抗体[EPR12441(B)]
参阅全部 KLC1 一抗 -
描述
兔单克隆抗体[EPR12441(B)] to KLC1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- U87-MG, 293T, SH-SY5Y, and HeLa whole cell lysate (ab150035); Human brain tissue; U87-MG and HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12441(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab174273于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 61-70 kDa (predicted molecular weight: 65 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/50 - 1/500.
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IP |
1/10 - 1/100.
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说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 61-70 kDa (predicted molecular weight: 65 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/50 - 1/500. |
IP
1/10 - 1/100. |
靶标
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功能
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity. -
组织特异性
Found in a variety of tissues. Mostly abundant in brain and spine. -
序列相似性
Belongs to the kinesin light chain family.
Contains 6 TPR repeats. -
翻译后修饰
Isoform I is phosphorylated on Ser-600. Isoform J is phosphorylated on Ser-631. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 3831 Human
- Entrez Gene: 16593 Mouse
- Entrez Gene: 171041 Rat
- Omim: 600025 Human
- SwissProt: Q07866 Human
- SwissProt: O88447 Mouse
- SwissProt: P37285 Rat
- Unigene: 20107 Human
see all -
别名
- Kinesin 2 60/70kDa antibody
- Kinesin light chain 1 antibody
- Kinesin2 antibody
see all
图片
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All lanes : Anti-KLC1 antibody [EPR12441(B)] (ab174273) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : KLC1 knockout HeLa cell lysate
Lane 3 : U-87 MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDaLanes 1-3: Merged signal (red and green). Green - ab174273 observed at 65 kDa. Red - loading control ab8245 observed at 36 kDa.
ab174273 Anti-KLC1 antibody [EPR12441(B)] was shown to specifically react with KLC1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265284 (knockout cell lysate ab257496) was used. Wild-type and KLC1 knockout samples were subjected to SDS-PAGE. ab174273 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling KLC1 with purified ab174273 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling KLC1 with ab174273 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-KLC1 antibody [EPR12441(B)] (ab174273) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 65 kDa -
Western blot analysis on immunoprecipitation pellet from SH-SY5Y cell lysate using ab174273 at 1/10 dilution.
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Immunofluorescent analysis of U87-MG cells labeling KLC1 with ab174273 at 1/50 dilution.
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Intracellular flow cytometric analysis of permeabilized HeLa cells labeling KLC1with ab174273 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab174273 被引用在 6 文献中.
- Yang X et al. Time-dependent alterations in the rat nigrostriatal system after intrastriatal injection of fibrils formed by α-Syn and tau fragments. Front Aging Neurosci 14:1049418 (2022). PubMed: 36518823
- Giovarelli M et al. Drp1 overexpression induces desmin disassembling and drives kinesin-1 activation promoting mitochondrial trafficking in skeletal muscle. Cell Death Differ 27:2383-2401 (2020). PubMed: 32042098
- Franchini DM et al. Microtubule-Driven Stress Granule Dynamics Regulate Inhibitory Immune Checkpoint Expression in T Cells. Cell Rep 26:94-107.e7 (2019). PubMed: 30605689
- Moamer A et al. A role for kinesin-1 subunits KIF5B/KLC1 in regulating epithelial mesenchymal plasticity in breast tumorigenesis. EBioMedicine 45:92-107 (2019). PubMed: 31204277
- Malikov V & Naghavi MH Localized Phosphorylation of a Kinesin-1 Adaptor by a Capsid-Associated Kinase Regulates HIV-1 Motility and Uncoating. Cell Rep 20:2792-2799 (2017). PubMed: 28930676
- Wilson MH & Holzbaur EL Nesprins anchor kinesin-1 motors to the nucleus to drive nuclear distribution in muscle cells. Development 142:218-28 (2015). PubMed: 25516977