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Treatment of DNA with sodium bisulfite leads to the deamination of cytosine residues and converts them to uracil, while 5-mC residues remain the same. These changes in the DNA sequence provide information about the methylation status of a DNA region with single-nucleotide resolution when detected in subsequent sequencing analysis.
Our bisulfite conversion kits have been optimized to accurately (>99%) convert unmethylated cytosine into uracil. With the ability to use a broad range of sample material, our range of bisulfite conversion kits give you the flexibility needed for your methylation studies.
Use our table below to select the right bisulfite kit for you
Conversion Kit - Whole Cell
|Fast Bisulfite |
|DNA Isolation & Bisulfite Conversion Kit||Bisulfite-Seq High Sensitivity Kit||Post-Bisulfite DNA Library Preparation Kit|
|Product principle||Direct bisulfite conversion from cells or tissue||Bisulfite conversion of purified DNA||DNA isolation and bisulfite conversion||Simultaneous bisulfite conversion and fragmentation for Illumina® sequencing||Fragmentation for Illumina® |
sequencing after bisulfite conversion
|Sample input||Cultured cells, tissue samples, embryonic cells / oocytes||Purified DNA||Serum, plasma and body fluids||Purified DNA||Purified bisulfite - converted DNA|
|Minimum sample amount||100 cells / 1 µg tissue||0.2 ng DNA||50 pg DNA / 20 cells||0.5 ng DNA||1 ng bisulfite-converted DNA|
|Protocol Time||3 hours||30 minutes||2.5 hours||6.5 hours||5 hours|