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What is SimpleStep ELISA?
SimpleStep ELISA® kits are sandwich ELISA assays that we have developed to be as simple and effective as possible. Unlike traditional sandwich ELISAs, which take more than three hours to run, SimpleStep ELISA kits generate data in just 90 minutes, without compromising on performance.
How does SimpleStep ELISA work?
A conventional sandwich ELISA uses a pair of antibodies that bind to at least two distinct sites on the target protein. The capture antibody is pre-coated onto the well of a microplate and selectively binds to the target protein. After a wash step, the detector antibody is added and binds to a second site on the target protein to form a sandwich complex.
SimpleStep ELISA kits streamline this process by using a homogeneous format wherein the antibody-analyte sandwich complex is formed in solution in a single step. In just one incubation and wash step, the complete sandwich complex forms in the well and is anchored to the plate with an immunoaffinity tag (figure 1).
Figure 1. With SimpleStep ELISA kits, an analyte-capture and detector antibody sandwich complex is formed in solution, which binds to the microplate via an affinity tag attached to the capture antibody in the sandwich pair. See the video of how SimpleStep ELISA works.
Uncompromising on sensitivity
SimpleStep ELISA kits drastically reduce assay times without compromising on performance. We compared the sensitivity of SimpleStep ELISA with that of the next best competitor brand of ELISA kits for 75 popular human and mouse proteins. In 56 out of 75 cases, SimpleStep ELISA shows superior sensitivity (figure 2).
Figure 2. We compared SimpleStep ELISA kit sensitivity with a competitor assay across 75 popular protein targets and calculated the ratios. SimpleStep ELISA was more sensitive on average than a competitor ELISA kit.
Tested in biological samples
Every SimpleStep ELISA kit is validated using multiple biological samples for assay specificity. All secreted serum or plasma-based targets are tested and fall within World Health Organization blood reference ranges. When available, the SimpleStep ELISA kits are calibrated against a known NIBSC international standards and includes a conversion factor for data comparison.
Wide range of analytes
We currently have SimpleStep ELISA kits to almost 500 targets, including popular proteins like PD-L1, GFP, and fibrinogen, and are continually adding more to the catalog.