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ELISA kits to mouse targets may be less reproducible, specific and sensitive when compared to ELISA kits to other species’ targets due to the use of batch-specific polyclonal antibodies. Monoclonal antibodies are preferred for consistent ELISA performance; however, the use of a mouse monoclonal antibody to mouse targets may generate an unacceptable level of background resulting in a less sensitive assay.
Many SimpleStep ELISA kits to mouse targets use RabMAb® antibody technology, Abcam’s patented rabbit monoclonal technology, to achieve greater sensitivity and specificity. Rabbit monoclonal antibodies have the enhanced affinity of a rabbit host antibody coupled with the specificity that is the hallmark of monoclonal antibodies. Most RabMAb antibodies are recombinant which means that greater batch-to-batch consistency in quality and performance.
During the development of a SimpleStep ELISA, multiple rabbit monoclonal antibody clones are screened for optimal performance as both capture and detector antibodies. As a result, SimpleStep ELISA frequently exhibit improved assay performance with greater sensitivity than the leading competitors:
Figure 1. Mouse IL-5 SimpleStep ELISA kit (ab204523) is more sensitive than the leading competitor.
Figure 2. Mouse IL-1 beta SimpleStep ELISA kit (ab197742) is more sensitive than the leading competitor.
In addition to improvements in sensitivity, SimpleStep ELISA kits offers these additional benefits to the researcher: