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SimpleStep ELISA kit uses a semi-homogeneous protocol where sample and antibodies are added in one step. The use of a semi-homogeneous format improves sensitivity when compared to sequential sandwich ELISA format.
The antibody sandwich is rapidly formed in solution before binding to the plate, meaning that only one hour incubation of the sample and the antibodies is required. For example, CXCL16 SimpleStep ELISA kit has an increased sensitivity compared to a conventional 3-step ELISA kit using a homogenous protocol (Figure 1).
Figure 1: Standard comparison between Human CXCL16 SimpleStep ELISA kit (ab187397) and a standard sequential ELISA kit.
Similarly, increased sensitivity was seen for Enolase 1 (ENO1) (Figure 2).