概述

  • 产品名称Anti-Kisspeptin抗体
    参阅全部 Kisspeptin 一抗
  • 描述
    兔多克隆抗体to Kisspeptin
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide near the N terminus of the full-length mouse KISS1 protein.

  • 阳性对照
    • Jurkat cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab19028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 15 kDa.Can be blocked with Kisspeptin peptide (ab37702).

靶标

  • 功能Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood.
  • 组织特异性Very high expression in placenta, with the next highest level in testis and moderate levels in pancreas, liver, small intestine and brain at much lower levels. Expression levels increased in both early placentas and molar pregnancies and are reduced in choriocarcinoma cells. Expressed at higher levels in first trimester trophoblasts than at term of gestation, but only expressed in the villous trophoblast.
  • 序列相似性Belongs to the KISS1 family.
  • 翻译后修饰Processed by MMP2 and MMP9.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KISS 1 antibody
    • KiSS 1 metastasis suppressor antibody
    • Kiss1 antibody
    • KISS1_HUMAN antibody
    • Kisspeptin 1 antibody
    • Kisspeptin 10 antibody
    • Kisspeptin 13 antibody
    • Kisspeptin 14 antibody
    • Kisspeptin antibody
    • Kisspeptin-1 antibody
    • Kisspeptin-10 antibody
    • Kisspeptin-54 antibody
    • Kisspeptin1 antibody
    • Malignant melanoma metastasis suppressor antibody
    • Metastasis suppressor KiSS 1 antibody
    • Metastasis suppressor KiSS1 antibody
    • Metastin antibody
    • MGC39258 antibody
    see all

Anti-Kisspeptin antibody 图像

  • Anti-Kisspeptin antibody (ab19028) at 1 µg/ml + Jurkat cell lysate (30ug)

    Predicted band size : 15 kDa
    Observed band size : 15,35 kDa (why is the actual band size different from the predicted?)


  • Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 15 kDa


    Exposure time : 2 minutes

    This image is courtesy of an Abreview submitted by Dr Aparna Mitra

    See Abreview

Anti-Kisspeptin antibody (ab19028)参考文献

This product has been referenced in:
  • Fernandois D  et al. Kisspeptin is involved in ovarian follicular development during aging in rats. J Endocrinol 228:161-70 (2016). Read more (PubMed: 26698566) »
  • Hua W  et al. Analysis of the serum concentrations of kisspeptin and neurokinin B in the geese during reproductive cycle and their localisation in the ovary. Anim Reprod Sci 151:78-84 (2014). Read more (PubMed: 25282553) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (brain)
Specification brain
Fixative Methanol
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Nitsan Kozlovsky

Verified customer

提交于 Oct 01 2012

Thank you for contacting us.
We would be interested in this information. I have therefore generated the testing discount as below:
testing discount: xxxxxx expiration date: 14-Nov-12
The code becomes active once the customer has submitte...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Breast cancer cells)
Loading amount 20 µg
Specification Breast cancer cells
Treatment overexpressing DNAJB6
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Aparna Mitra

Verified customer

提交于 Jul 11 2008

Thank you for your enquiry. I have been informed by our source for this antibody that the proprietary peptide used to generate this antibody is from the N-terminus of the secreted protein, so it will recognize the secreted as well as the intracellular ...

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I'm sorry about the confusion. The NP_839991 (PubMed/Search protein) provides the mouse Kisspeptin amino acid sequence. I've now included this information below. Based on this, amino acid 14-27/the immunogen sequence would be "cvatygeplakvap". ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"