概述

  • 产品名称Anti-Kir2.2抗体[S24-1]
    参阅全部 Kir2.2 一抗
  • 描述
    小鼠单克隆抗体[S24-1] to Kir2.2
  • 特异性Does not cross react with Kir2.1 and Kir2.3
  • 经测试应用适用于: IHC-P, IHC-Fr, WB, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Fusion protein: LVENKFLLPS ANSFCYENEL AFLSRDEEDE VATDRDGRSP QPEHDFDRLQ ASSGALERPY RRESEI, corresponding to amino acids 362-427 of Rat Kir2.2 (NP_446433).

  • 阳性对照
    • Rat brain lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号S24-1
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab84821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 48 kDa.
ICC/IF Use a concentration of 1 - 10 µg/ml.
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.
  • 序列相似性Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ12 subfamily.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP sensitive inward rectifier potassium channel 12 antibody
    • ATP-sensitive inward rectifier potassium channel 12 antibody
    • hIRK antibody
    • Human high conductance inward rectifier potassium channel alpha antibody
    • Inward rectifier K antibody
    • Inward rectifier K(+) channel Kir2.2 antibody
    • Inward rectifier K(+) channel Kir2.2v antibody
    • inwardly rectifying subfamily J member 12 antibody
    • IRK-2 antibody
    • IRK12_HUMAN antibody
    • IRK2 antibody
    • KCNJ12 antibody
    • kcnj12x antibody
    • KCNJN1 antibody
    • Kir2.2v antibody
    • Potassium channel antibody
    • Potassium channel inwardly rectifying subfamily J member 12 antibody
    • Potassium inwardly rectifying channel subfamily J member 12 antibody
    • Potassium inwardly rectifying channel,subfamily J inhibitor 1 antibody
    see all

Anti-Kir2.2 antibody [S24-1] 图像

  • All lanes : Anti-Kir2.2 antibody [S24-1] (ab84821)

    Lane 1 : Molecular weight marker
    Lane 2 : Cell lysates prepared from human cell lines A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3 and T98G at 10 µg


    Predicted band size : 48 kDa
  • ab84821 staining Kir2.2 in human hippocampus tissue section by IHC-P. Bouin's fixed and paraffin embedded tissue sections were used.

  • Overlay histogram showing SH-SY5Y cells stained with ab84821 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84821, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Kir2.2 antibody [S24-1] (ab84821)参考文献

This product has been referenced in:
  • Sivagangabalan G  et al. Regional ion channel gene expression heterogeneity and ventricular fibrillation dynamics in human hearts. PLoS One 9:e82179 (2014). WB ; Human . Read more (PubMed: 24427266) »

See 1 Publication for this product

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