Anti-Kinetochore抗体(ab85996)

概述

  • 产品名称Anti-Kinetochore抗体
    参阅全部 Kinetochore 一抗
  • 描述
    兔多克隆抗体to Kinetochore
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Xenopus tropicalis , Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide, corresponding to a region within residues 375 and 425 of human Kinetochore (NP_055523.1).

  • 阳性对照
    • HeLa whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab85996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2-5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 250 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性The kinetochore is the protein structure in eukaryotes which assembles on the centromere and links the chromosome to microtubule polymers from the mitotic spindle during mitosis and meiosis. The kinetochore contains two regions: an inner kinetochore, which is tightly associated with the centromere DNA; and an outer kinetochore, which interacts with microtubules. Even the simplest kinetochores consist of more than 45 different proteins, many of which are conserved throughout eukaryote species.
    • 细胞定位Cytoplasmic and Nuclear
    • 数据库链接
    • 别名
      • HsROD antibody
      • Kinetochore associated 1 antibody
      • KNTC1 antibody
      • ROD antibody
      • Rough Deal homolog, centromere/kinetochore protein antibody
      see all

    Anti-Kinetochore antibody 图像

    • All lanes : Anti-Kinetochore antibody (ab85996) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 250 kDa
      Observed band size : 250 kDa
      Additional bands at : 115 kDa,175 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 10 seconds
    • Detection of Kinetochore by Western Blot of Immunprecipitate.
      ab85996, at 1 µg/ml, staining Kinetochore in HeLa whole cell lysate immunoprecipitated using ab8599 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 10 seconds.

    Anti-Kinetochore antibody (ab85996)参考文献

    ab85996 has not yet been referenced specifically in any publications.

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