The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 117 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 5 µg/ml.
Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL).
Distributed throughout the CNS but is highly enriched in subsets of neurons.
Defects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10) [MIM:604187]. An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
Belongs to the kinesin-like protein family. Kinesin subfamily. Contains 1 kinesin-motor domain.
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton. Concentrated in the cell body of the neurons, particularly in the perinuclear region.
Human Brain, Cortex tissue (formalin-fixed, paraffin-embedded) stained with ab118534 at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-Kinesin 5A antibody (ab118534)
Lane 1 : Anti-Kinesin 5A antibody (ab118534) at 0.5 µg/ml Lane 2 : Anti-Kinesin 5A antibody (ab118534) at 1 µg/ml
Lane 1 : K562 cell lysate Lane 2 : K562 cell lysate