Anti-Kinesin 5 A + B + C抗体(ab62104)

Abreviews (4)Q&A (1)Specific References (1)
Customer reviews and FAQs

概述

  • 产品名称
    Anti-Kinesin 5 A + B + C抗体
    参阅全部 Kinesin 5 A + B + C 一抗
  • 描述
    兔多克隆抗体to Kinesin 5 A + B + C
  • 经测试应用
    适用于: ICC/IF, IP, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Raised against a 20 amino acid peptide from near the carboxy terminus of human kinesin 5 (GenBank accession no. Q12840).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab62104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500.
IP 1/400. (see Abreview)
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 116 kDa (predicted molecular weight: 110 kDa).
IHC-Fr Use at an assay dependent concentration.

靶标

  • 相关性
    The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. KIF5 has at least three known isoforms; this antibody recognizes all three. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias.
  • 细胞定位
    Uniformly distributed between soma and neurites in hippocampal neurons
  • 数据库链接
    • 别名
      • KIF5A antibody
      • KIF5B antibody
      • KIF5C antibody
      • Kinesin family member 5A antibody
      • Kinesin family member 5B antibody
      • Kinesin family member 5C antibody
      • KINH antibody
      • KINN antibody
      • NKHC antibody
      • NKHC2 antibody
      see all

    Anti-Kinesin 5 A + B + C antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinesin 5 A + B + C antibody (ab62104)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinesin 5 A + B + C antibody (ab62104)
      Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling Kinesin 5 A + B + C with Anti-Kinesin 5 A + B + C antibody (ab62104) at 5μg/ml.
    • Western blot - Kinesin 5 A + B + C antibody (ab62104)
      Western blot - Kinesin 5 A + B + C antibody (ab62104)
      Lane 1 : Anti-Kinesin 5 A + B + C antibody (ab62104) at 0.5 µg/ml
      Lane 2 : Anti-Kinesin 5 A + B + C antibody (ab62104) at 1 µg/ml

      Lane 1 : K562 cell lysate
      Lane 2 : K562 cell lysate


      Predicted band size : 110 kDa
      Observed band size : 116 kDa (why is the actual band size different from the predicted?)
    • Immunocytochemistry - Kinesin 5 A + B + C antibody (ab62104)
      Immunocytochemistry - Kinesin 5 A + B + C antibody (ab62104)

      ab62104 at 5 µg/ml staining Kinesin 5 A + B + C in human K562 cells by Immunocytochemistry.

    • Immunocytochemistry/ Immunofluorescence-Anti-Kinesin 5 A + B + C antibody(ab62104)
      Immunocytochemistry/ Immunofluorescence-Anti-Kinesin 5 A + B + C antibody(ab62104)
      Immunofluorescence of KIF5 in K562 cells using ab62104 at 20 ug/ml.

    Anti-Kinesin 5 A + B + C antibody (ab62104)参考文献

    This product has been referenced in:
    • Su B  et al. Brain-derived neurotrophic factor (BDNF)-induced mitochondrial motility arrest and presynaptic docking contribute to BDNF-enhanced synaptic transmission. J Biol Chem 289:1213-26 (2014). Rat . Read more (PubMed: 24302729) »

    See 1 Publication for this product

    发表研究结果有使用 ab62104？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    Immunohistochemistry (Frozen sections) abreview for Anti-Kinesin 5 A + B + C antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Sample
    Mouse Tissue sections (spinal cord)
    Specification
    spinal cord
    Permeabilization
    Yes - triton 0.3%
    Fixative
    Lana's Fix
    Read More
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    Abcam user community

    Verified customer

    提交于 Jul 16 2013

    I have a techinical question about Ab Anti kinesin A+B+C (ab62104) antibody.
    I just bought it. I noticed that in the Abreview about western blot the customer reported
    the use of Non-reduced Denaturing (4-20% Tris-Glycine Gel) gel.
    However,...

    Read More

    We publish all the data we receive in Abreviews from customers, in same way they submit to us. Customer have used non reducing conditions however we have tested this antibody under reducing/ denaturing conditions so this antibody is fully guaranteed fo...

    Read More

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Kinesin 5 A + B + C antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa Cells)
    Specification
    HeLa Cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton
    Blocking step
    BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 4°C
    Read More
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    Abcam user community

    Verified customer

    提交于 Oct 21 2010

    Immunoprecipitation abreview for Anti-Kinesin 5 A + B + C antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Rat Tissue lysate - whole (brain, embryonic E18)
    Total protein in input
    1000 µg
    Specification
    brain, embryonic E18
    Immuno-precipitation step
    Protein G
    Read More
    Username

    Lena

    Verified customer

    提交于 Sep 10 2010

    Western blot abreview for Anti-Kinesin 5 A + B + C antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain Lysate loaded onto a 5-20% sucrose gradient)
    Loading amount
    10 µg
    Specification
    Brain Lysate loaded onto a 5-20% sucrose gradient
    Gel Running Conditions
    Non-reduced Denaturing (4-20% Tris-Glycine Gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Abcam user community

    Verified customer

    提交于 Nov 26 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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