- 数据表
- 具体的参考文献 (4)
- 实验方案
概述
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产品名称Anti-Kinesin 5 A + B + C抗体
参阅全部 Kinesin 5 A + B + C 一抗 -
描述兔多克隆抗体to Kinesin 5 A + B + C
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宿主Rabbit
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经测试应用适用于: ICC/IF, IP, WB, IHC-Frmore details
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种属反应性与反应: Mouse, Rat, Human
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免疫原
Raised against a 20 amino acid peptide from near the carboxy terminus of human kinesin 5 (GenBank accession no. Q12840).
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab62104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/500. | |
| IP | 1/400. (see Abreview) | |
| WB | Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 116 kDa (predicted molecular weight: 110 kDa). | |
| IHC-Fr | Use at an assay dependent concentration. |
靶标
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相关性The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. KIF5 has at least three known isoforms; this antibody recognizes all three. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias.
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细胞定位Uniformly distributed between soma and neurites in hippocampal neurons
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数据库链接
- Entrez Gene: 3798 Human
- Entrez Gene: 3799 Human
- Entrez Gene: 3800 Human
- SwissProt: O60282 Human
- SwissProt: P33176 Human
- SwissProt: Q12840 Human
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别名
- KIF5A antibody
- KIF5B antibody
- KIF5C antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinesin 5 A + B + C antibody (ab62104)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse brain tissue staining Kinesin 5 A + B + C with ab62104 at 20µg/ml -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinesin 5 A + B + C antibody (ab62104)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling Kinesin 5 A + B + C with Anti-Kinesin 5 A + B + C antibody (ab62104) at 5μg/ml. -
Western blot - Anti-Kinesin 5 A + B + C antibody (ab62104)Lane 1 : Anti-Kinesin 5 A + B + C antibody (ab62104) at 0.5 µg/ml
Lane 2 : Anti-Kinesin 5 A + B + C antibody (ab62104) at 1 µg/ml
All lanes : K562 cell lysate
Predicted band size: 110 kDa
Observed band size: 116 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry - Anti-Kinesin 5 A + B + C antibody (ab62104)ab62104 at 5 µg/ml staining Kinesin 5 A + B + C in human K562 cells by Immunocytochemistry.
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Immunocytochemistry/ Immunofluorescence - Anti-Kinesin 5 A + B + C antibody (ab62104)Immunofluorescence of KIF5 in K562 cells using ab62104 at 20 ug/ml.
实验方案
文献
This product has been referenced in:
- Thillaiappan NB et al. Ca2+signals initiate at immobile IP3receptors adjacent to ER-plasma membrane junctions. Nat Commun 8:1505 (2017). Read more (PubMed: 29138405) »
- Farías GG et al. BORC/kinesin-1 ensemble drives polarized transport of lysosomes into the axon. Proc Natl Acad Sci U S A 114:E2955-E2964 (2017). Read more (PubMed: 28320970) »
客户评价及客户问答
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