Anti-Kindlin 2抗体(ab74030)

概述

  • 产品名称Anti-Kindlin 2抗体
    参阅全部 Kindlin 2 一抗
  • 描述
    兔多克隆抗体to Kindlin 2
  • 经测试应用适用于: IP, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human Kindlin 2.

    (Peptide available as ab74029.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: HT 1080 Whole Cell, SAOS-2 Whole Cell, Human Testis Tissue and HeLa Nuclear

性能

应用

Our Abpromise guarantee covers the use of ab74030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).Can be blocked with Human Kindlin 2 peptide (ab74029).
ICC/IF 1/100.

靶标

  • 功能Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.
  • 组织特异性Ubiquitous. Found in numerous tumor tissues.
  • 序列相似性Belongs to the kindlin family.
    Contains 1 FERM domain.
    Contains 1 PH domain.
  • 结构域The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.
  • 细胞定位Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Within actin stress fibers at cell-ECM focal adhesion sites.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ34213 antibody
    • FLJ44462 antibody
    • UNC112 antibody
    • FERM2_HUMAN antibody
    • Fermitin family homolog 2 antibody
    • Fermt2 antibody
    • KIND2 antibody
    • Kindlin-2 antibody
    • MIG-2 antibody
    • MIG2 antibody
    • Mitogen-inducible gene 2 protein antibody
    • PH domain-containing family C member 1 antibody
    • Pleckstrin homology domain containing family C (with FERM domain) member 1 antibody
    • Pleckstrin homology domain-containing family C member 1 antibody
    • PLEKHC1 antibody
    • UNC112B antibody
    see all

Anti-Kindlin 2 antibody 图像

  • All lanes : Anti-Kindlin 2 antibody (ab74030) at 1 µg/ml

    Lane 1 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
    Lane 2 : SAOS-2 (sarcoma osteogenic) Whole Cell lysate
    Lane 3 : Human testis tissue lysate - total protein (ab30257)
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 78 kDa


    Exposure time : 8 minutes
  • Kindlin 2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Kindlin 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab74030.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 78kDa; Kindlin 2


  • Predicted band size : 78 kDa

    This image is courtesy of an abreview submitted by Ana Kasirer-Friede,UCSD , United States

    The kindlin 2 antibody (ab74030) was tested in lysate obtained from CHO cells either expressing endogenous levels of kindlin 2 (lane1)and transfected CHO cells in which flag-tagged kindlin2 was overexpressed (lane 2). A specific band was at the expected molecular weight of approximately 72 kDa (+/- Flag-tag). ß-actin was used to show loading in the two lanes.

    See Abreview

  • ab74030 staining Kindlin 2 in HeLa cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using Triton, then incubated with ab74030 at a 1/100 dilution for 1 hour at 25°C. The secondary used was a Cy3-AffiniPure F(ab')2 fragment goat anti-rabbit monoclonal, used at a 1/200 dilution.

    See Abreview

Anti-Kindlin 2 antibody (ab74030)参考文献

ab74030 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293T cells)
Loading amount 25 µg
Specification HEK293T cells
Gel Running Conditions Reduced Denaturing (4-12% SDS-PAGE gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jan 28 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mouse Embryonic Fibroblast (MEF))
Specification Mouse Embryonic Fibroblast (MEF)
Fixative Paraformaldehyde
Permeabilization Yes - triton
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Abcam user community

Verified customer

提交于 Sep 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cell line ( cervical cancer))
Specification HeLa cell line ( cervical cancer)
Fixative Paraformaldehyde
Permeabilization Yes - triton
Username

Abcam user community

Verified customer

提交于 Sep 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (CHO cell)
Loading amount 40 µg
Specification CHO cell
Treatment +/- transfect human kindlin2
Gel Running Conditions Reduced Denaturing (7.5)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Dr. Ana Kasirer-Friede

Verified customer

提交于 Jul 19 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skeletal muscle)
Specification Skeletal muscle
Fixative tried different conditions
Permeabilization Yes - 0.5% tx-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
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Abcam user community

Verified customer

提交于 Oct 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"