Anti-Kindlin 2抗体(ab74030)

概述

  • 产品名称Anti-Kindlin 2抗体
    参阅全部 Kindlin 2 一抗
  • 描述
    兔多克隆抗体to Kindlin 2
  • 经测试应用适用于: IP, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human Kindlin 2.

    (Peptide available as ab74029.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: HT 1080 Whole Cell, SAOS-2 Whole Cell, Human Testis Tissue and HeLa Nuclear

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab74030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).Can be blocked with Human Kindlin 2 peptide (ab74029).
ICC/IF 1/100.

靶标

  • 功能Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.
  • 组织特异性Ubiquitous. Found in numerous tumor tissues.
  • 序列相似性Belongs to the kindlin family.
    Contains 1 FERM domain.
    Contains 1 PH domain.
  • 结构域The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.
  • 细胞定位Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Within actin stress fibers at cell-ECM focal adhesion sites.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ34213 antibody
    • FLJ44462 antibody
    • UNC112 antibody
    • FERM2_HUMAN antibody
    • Fermitin family homolog 2 antibody
    • Fermt2 antibody
    • KIND2 antibody
    • Kindlin-2 antibody
    • MIG-2 antibody
    • MIG2 antibody
    • Mitogen-inducible gene 2 protein antibody
    • PH domain-containing family C member 1 antibody
    • Pleckstrin homology domain containing family C (with FERM domain) member 1 antibody
    • Pleckstrin homology domain-containing family C member 1 antibody
    • PLEKHC1 antibody
    • UNC112B antibody
    see all

Anti-Kindlin 2 antibody 图像

  • All lanes : Anti-Kindlin 2 antibody (ab74030) at 1 µg/ml

    Lane 1 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
    Lane 2 : SAOS-2 (sarcoma osteogenic) Whole Cell lysate
    Lane 3 : Human testis tissue lysate - total protein (ab30257)
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 78 kDa


    Exposure time : 8 minutes
  • Kindlin 2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Kindlin 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab74030.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 78kDa; Kindlin 2


  • Predicted band size : 78 kDa

    This image is courtesy of an abreview submitted by Ana Kasirer-Friede,UCSD , United States

    The kindlin 2 antibody (ab74030) was tested in lysate obtained from CHO cells either expressing endogenous levels of kindlin 2 (lane1)and transfected CHO cells in which flag-tagged kindlin2 was overexpressed (lane 2). A specific band was at the expected molecular weight of approximately 72 kDa (+/- Flag-tag). ß-actin was used to show loading in the two lanes.

    See Abreview

  • ab74030 staining Kindlin 2 in HeLa cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using Triton, then incubated with ab74030 at a 1/100 dilution for 1 hour at 25°C. The secondary used was a Cy3-AffiniPure F(ab')2 fragment goat anti-rabbit monoclonal, used at a 1/200 dilution.

    See Abreview

Anti-Kindlin 2 antibody (ab74030)参考文献

ab74030 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293T cells)
Loading amount 25 µg
Specification HEK293T cells
Gel Running Conditions Reduced Denaturing (4-12% SDS-PAGE gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jan 28 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mouse Embryonic Fibroblast (MEF))
Specification Mouse Embryonic Fibroblast (MEF)
Fixative Paraformaldehyde
Permeabilization Yes - triton
Username

Abcam user community

Verified customer

提交于 Sep 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cell line ( cervical cancer))
Specification HeLa cell line ( cervical cancer)
Fixative Paraformaldehyde
Permeabilization Yes - triton
Username

Abcam user community

Verified customer

提交于 Sep 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (CHO cell)
Loading amount 40 µg
Specification CHO cell
Treatment +/- transfect human kindlin2
Gel Running Conditions Reduced Denaturing (7.5)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Dr. Ana Kasirer-Friede

Verified customer

提交于 Jul 19 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skeletal muscle)
Specification Skeletal muscle
Fixative tried different conditions
Permeabilization Yes - 0.5% tx-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Oct 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"