概述

  • 产品名称Anti-KIFC3抗体
    参阅全部 KIFC3 一抗
  • 描述
    兔多克隆抗体to KIFC3
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog, Orangutan
  • 免疫原

    Synthetic peptide: SQEHLEWEPA CQTPQPSARA HSAPSSGTSS RPGSIRRKLQ PSGKSRPLPV , corresponding to C terminal amino acids 784 - 833 of Human KIFC3 (NP_005541)

  • 阳性对照
    • MCF7 cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab80449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

靶标

  • 功能Minus-end microtubule-dependent motor protein. Involved in apically targeted transport (By similarity). Required for zonula adherens maintenance.
  • 序列相似性Belongs to the kinesin-like protein family.
    Contains 1 kinesin-motor domain.
  • 细胞定位Cell junction > adherens junction. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic vesicle membrane. Apical cell membrane. On membrane organelles immediately beneath the apical plasma membrane of renal tubular epithelial cells. Localized in the distal tubules and loops of Henle in the kidney, but not in the proximal tubules or the glomeruli, with stronger staining in the apical area of these epithelial cells (By similarity). Localizes along zonula adherens only at mature cell-cell contacts.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686D23201 antibody
    • FLJ34694 antibody
    • Kifc3 antibody
    • KIFC3_HUMAN antibody
    • Kinesin family member C3 antibody
    • Kinesin-like protein KIFC3 antibody
    see all

Anti-KIFC3 antibody 图像

  • Anti-KIFC3 antibody (ab80449) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 93 kDa
    Observed band size : 93 kDa
    Additional bands at : 36 kDa,40 kDa,48 kDa. We are unsure as to the identity of these extra bands.

Anti-KIFC3 antibody (ab80449)参考文献

ab80449 has not yet been referenced specifically in any publications.

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