重组Anti-KIFC1抗体[11445] (ab172620)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [11445] to KIFC1
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-KIFC1抗体[11445]
参阅全部 KIFC1 一抗 -
描述
兔单克隆抗体[11445] to KIFC1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human KIFC1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9BW19 -
阳性对照
- WB: HeLa, 293T, HepG2 and HAP1 cell lysates IHC-P: Human tonsil tissue, cervical carcinoma, stomachICC/IF: HeLa cells Flow Cyt (intra): HeLa cells IP: Jurkat whole cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
11445 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab172620于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/20.
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WB |
1/10000 - 1/50000. Predicted molecular weight: 74 kDa.
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ICC/IF |
1/50 - 1/100.
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IP |
1/10 - 1/100.
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IHC-P |
1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For unpurified use at 1/50 - 1/100. See IHC antigen retrieval protocols. |
说明 |
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Flow Cyt (Intra)
1/20. |
WB
1/10000 - 1/50000. Predicted molecular weight: 74 kDa. |
ICC/IF
1/50 - 1/100. |
IP
1/10 - 1/100. |
IHC-P
1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use at 1/50 - 1/100. See IHC antigen retrieval protocols. |
靶标
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功能
Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles. -
序列相似性
Belongs to the kinesin-like protein family. NCD subfamily.
Contains 1 kinesin-motor domain. -
细胞定位
Nucleus. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Early endosome. Associated with nucleus during interphase, centrosomes in early and spindle in later mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 3833 Human
- Omim: 603763 Human
- SwissProt: Q9BW19 Human
- Unigene: 436912 Human
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别名
- HSET antibody
- HSET KNSL2 antibody
- KIF C1 antibody
see all
图片
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Anti-KIFC1 antibody [11445] (ab172620) at 1/50000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa -
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: KIFC1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab172620 (purified) observed at 74 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab172620 was shown to specifically react with KIFC1 in wild-type cells as signal was lost in KIFC1 knockout cells. Wild-type and KIFC1 knockout samples were subjected to SDS-PAGE. Ab172620 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 0.228 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling KIFC1 with purified ab172620 at 1/16000 dilution (0.01 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling KIFC1 with purified ab172620 at 1/50 dilution (4.0 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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ab172620 (purified) at 1/20 dilution (1ug) immunoprecipitating KIFC1 in Jurkat whole cell lysates.
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates 10ug
Lane 2 (+): ab172620 & Jurkat whole cell lysates
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab172620 in Jurkat whole cell lysates
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling KIFC1 with purified ab172620 at 1/20 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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All lanes : Anti-KIFC1 antibody [11445] (ab172620) at 1/10000 dilution ((unpurified))
Lane 1 : HeLa lysate
Lane 2 : 293T lysate
Lane 3 : HepG2 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 74 kDa -
Western blot analysis on immunoprecipitation pellet from 293T cell lysate, labeling KIFC1 with ab172620 (unpurified) at 1/10 dilution.
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue laneling KIFC1 with ab172620 (unpurified) at 1/50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling KIFC1 with ab172620 (unpurified) at 1/50 dilution.
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Immunofluorescent staining of HeLa cells labeling KIFC1 with ab172620 (unpurified) at 1/50 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab172620 被引用在 16 文献中.
- Liang LY & Li GS The Roles of KIFC1 in the Development of Osteosarcoma: Characterization of Potential Therapeutic Targets. Comput Math Methods Med 2022:5039134 (2022). PubMed: 35479190
- Fan G et al. The ATM and ATR kinases regulate centrosome clustering and tumor recurrence by targeting KIFC1 phosphorylation. Nat Commun 12:20 (2021). PubMed: 33397932
- Kostecka LG et al. High KIFC1 expression is associated with poor prognosis in prostate cancer. Med Oncol 38:47 (2021). PubMed: 33760984
- Begemann D et al. Androgens modify therapeutic response to cabazitaxel in models of advanced prostate cancer. Prostate 80:926-937 (2020). PubMed: 32542812
- Parvin A et al. Inhibition of kinesin motor protein KIFC1 by AZ82 induces multipolar mitosis and apoptosis in prostate cancer cell. Gene 760:144989 (2020). PubMed: 32717307