概述

  • 产品名称Anti-KIF7抗体
  • 描述
    兔多克隆抗体to KIF7
  • 经测试应用适用于: WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    预测可用于: Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide designed within residues: KRSLCSEGRQ APGNEDELHL APELLWLSPL TEGAPRTREE TRDLVHAPLP L, corresponding to amino acids 1225-1275 of Human KIF7 (NP_940927.2)

  • 阳性对照
    • HeLa or 293T cell lysate
  • 常规说明Shelf Life: 1 year from date of receipt

性能

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应用

Our Abpromise guarantee covers the use of ab95884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 151 kDa.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Acts as both a negative and positive regulator of sonic hedgehog (Shh) pathway, acting downstream of SMO. Negatively regulates the pathway by preventing inappropriate activation of the transcriptional activator GLI2 in the absence of ligand. Positively regulates the pathway by preventing the processing of the transcription factor GLI3 into its repressor form. Required for efficient localization of GLI3 to cilia in response to Shh. May also act as a ciliary motor.
    • 组织特异性Embryonic stem cells, melanotic melanoma and Jurkat T-cells.
    • 序列相似性Belongs to the kinesin-like protein family. KIF27 subfamily.
      Contains 1 kinesin-motor domain.
    • 细胞定位Cell projection > cilium. SMO is required for its accumulation within cilia. Moves from the cilia base to the cilia tip in response to activation of the Shh pathway.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EQYK340 antibody
      • kif7 antibody
      • KIF7_HUMAN antibody
      • kinesin family member 7 antibody
      • kinesin like protein KIF7 antibody
      • Kinesin-like protein kif7 antibody
      • UNQ340 antibody
      see all

    Anti-KIF7 antibody 图像

    • All lanes : Anti-KIF7 antibody (ab95884) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 151 kDa


      Exposure time : 30 seconds

    Anti-KIF7 antibody (ab95884)参考文献

    This product has been referenced in:
    • Ho J  et al. Downregulation of the gli transcription factors regulator Kif7 facilitates cell survival and migration of choriocarcinoma cells. PLoS One 9:e108248 (2014). WB ; Human . Read more (PubMed: 25265279) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Mouse Cell lysate - whole cell (MEF)
    Specification MEF
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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    提交于 Mar 03 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"