概述

  • 产品名称Anti-KIF5B抗体[H2]
    参阅全部 KIF5B 一抗
  • 描述
    小鼠单克隆抗体[H2] to KIF5B
  • 特异性ab23664 recognises the heavy chain of kinesin.
  • 经测试应用适用于: Electron Microscopy, IHC-FoFr, ICC, WB, IP, RIAmore details
  • 种属反应性
    与反应: Rat, Chicken, Sea Urchin
    预测可用于: all Mammals
  • 免疫原

    Full length protein corresponding to Cow KIF5B.

  • 表位The epitope recognised by ab23664 is located near the N terminus of Kinesin heavy chain.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 250mM Sodium chloride, 20mM PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号H2
  • 同种型IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab23664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Electron Microscopy
IHC-FoFr
ICC
WB
IP
RIA
  • 应用说明Electron Microscopy: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    IHC-F: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    RIA: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 109 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes.
    • 序列相似性Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
      Contains 1 kinesin motor domain.
    • 结构域Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
    • 细胞定位Cytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Conventional kinesin heavy chain antibody
      • KIF 5B antibody
      • KIF5B antibody
      • Kinesin 1 antibody
      • kinesin 1 (110-120kD) antibody
      • Kinesin 1 heavy chain antibody
      • Kinesin family member 5B antibody
      • Kinesin heavy chain antibody
      • kinesin, heavy chain, ubiquitous antibody
      • Kinesin-1 heavy chain antibody
      • Kinesin1 antibody
      • KINH antibody
      • KINH_HUMAN antibody
      • KNS 1 antibody
      • KNS antibody
      • KNS1 antibody
      • Ubiquitous kinesin heavy chain antibody
      • UKHC antibody
      see all

    Anti-KIF5B antibody [H2] (ab23664)参考文献

    This product has been referenced in:
    • Astanina K & Jacob R KIF5C, a kinesin motor involved in apical trafficking of MDCK cells. Cell Mol Life Sci 67:1331-42 (2010). IP . Read more (PubMed: 20094756) »
    • Zhang L  et al. {gamma}-Diketone Axonopathy: Analyses of Cytoskeletal Motors and Highways in CNS Myelinated Axons. Toxicol Sci 117:180-9 (2010). WB ; Rat . Read more (PubMed: 20554699) »

    See all 3 Publications for this product

    Product Wall

    Thank you for contacting Abcam regarding ab23664.

    I have run the BLAST search using the immunogen sequence for this antibody. I am only pulling up 100% with the heavy chain of kinesin-1. None of the other kinesins come up. I would predict th...

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    Thank you for your enquiry. The immunogen for this antibody was kinesin isolated from bovine brain. The antibody is described in detail in one of the references listed towards the bottom of the datasheet: Pfister KK, Wagner MC, Stenoien DL, Bra...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"