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Our Abpromise guarantee covers the use of ab5629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).Can be blocked with Human KIF5B peptide (ab41782).|
|IP||Use at an assay dependent concentration.|
ab5629 labelling KIF5B (green) in HeLa cells. Cells fixed in 3.2% paraformaldehyde and permeabilized with 0.2% Triton X-100 for 30 minutes at 37ºC were blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:250) overnight at 4°C. A fluorescently-conjugated Goat anti-rabbit IgG was used as the secondary antibody (1:2000). Images were taken on wide-field fluorescent microscope at 63X magnification.
Shows ab5629 used for a Western blot of Mouse neuronal cell extract.
ab5629 at 1/200 dilution staining the mouse oligodendrocyte cell line, Olineu, by immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy3 conjugated secondary antibody.
This image is courtesy of an Abreview submitted by Katarina Trajko on 22 February 2006.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"