概述

  • 产品名称Anti-KIF4A抗体[EPR5459]
    参阅全部 KIF4A 一抗
  • 描述
    兔单克隆抗体[EPR5459] to KIF4A
  • 经测试应用适用于: WB, Flow Cytmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • 阳性对照
    • Mouse thymus tissue lysate; HeLa cell lysate; 293T cell lysate; 293 cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 140 kDa).
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC,IHC-P or IP.
  • 靶标

    • 功能Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization.
    • 组织特异性Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.
    • 序列相似性Belongs to the kinesin-like protein family. Chromokinesin subfamily.
      Contains 1 kinesin-motor domain.
    • 细胞定位Nucleus matrix. Cytoplasm > cytoskeleton > spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Chromokinesin antibody
      • Chromokinesin-A antibody
      • Chromosome associated kinesin KIF4A antibody
      • Chromosome-associated kinesin KIF4A antibody
      • FLJ12530 antibody
      • FLJ12655 antibody
      • FLJ14204 antibody
      • FLJ20631 antibody
      • HSA271784 antibody
      • KIF4 antibody
      • KIF4 G1 antibody
      • KIF4A antibody
      • KIF4A_HUMAN antibody
      • KIF4G1 antibody
      • Kinesin family member 4A antibody
      see all

    Anti-KIF4A antibody [EPR5459] 图像

    • All lanes : Anti-KIF4A antibody [EPR5459] (ab124903) at 1/1000 dilution

      Lane 1 : Mouse thymus tissue lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 140 kDa
      Observed band size : 170 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeablized 293 cells using ab124903 at 1/10 dilution (red) or a rabbit IgG negative control (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-KIF4A antibody [EPR5459] (ab124903)参考文献

    This product has been referenced in:
    • Willemsen MH  et al. Involvement of the kinesin family members KIF4A and KIF5C in intellectual disability and synaptic function. J Med Genet 51:487-94 (2014). Read more (PubMed: 24812067) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"