概述

  • 产品名称Anti-KIF4A抗体
    参阅全部 KIF4A 一抗
  • 描述
    兔多克隆抗体to KIF4A
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Cow, Pig, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1182 and the C terminus (residue 1232) of human KIF4A (NP_036442.3)

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab72412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 140 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization.
  • 组织特异性Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.
  • 序列相似性Belongs to the kinesin-like protein family. Chromokinesin subfamily.
    Contains 1 kinesin-motor domain.
  • 细胞定位Nucleus matrix. Cytoplasm > cytoskeleton > spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Chromokinesin antibody
    • Chromokinesin-A antibody
    • Chromosome associated kinesin KIF4A antibody
    • Chromosome-associated kinesin KIF4A antibody
    • FLJ12530 antibody
    • FLJ12655 antibody
    • FLJ14204 antibody
    • FLJ20631 antibody
    • HSA271784 antibody
    • KIF4 antibody
    • KIF4 G1 antibody
    • KIF4A antibody
    • KIF4A_HUMAN antibody
    • KIF4G1 antibody
    • Kinesin family member 4A antibody
    see all

Anti-KIF4A antibody 图像

  • All lanes : Anti-KIF4A antibody (ab72412) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 140 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of KIF4A by Western Blot of Immunprecipitate.
    ab72412 at 1µg/ml staining KIF4A in HeLa whole cell lysate immunoprecipitated using ab72412 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).
    Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-KIF4A antibody (ab72412)参考文献

ab72412 has not yet been referenced specifically in any publications.

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