概述

  • 产品名称Anti-KIF4A抗体
    参阅全部 KIF4A 一抗
  • 描述
    兔多克隆抗体to KIF4A
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: LTLLQVASRQ KHLPKDTLLS PDSSFEYVPP KPKPSRVKEK FLEQSMDIED LKYCSEHSVN EHEDGDGDDD EGDDEEWKPT KL, corresponding to amino acids 994-1075 of Human KIF4A (UniProt ID: O95239).

  • 阳性对照
    • WB: RT-4 and U-251 MG cell lysates IHC-P: Human bone marrow tissue ICC/IF: Human cell line U-2 OS

性能

应用

Our Abpromise guarantee covers the use of ab122227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.
WB 1/250 - 1/500.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization.
  • 组织特异性Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.
  • 序列相似性Belongs to the kinesin-like protein family. Chromokinesin subfamily.
    Contains 1 kinesin-motor domain.
  • 细胞定位Nucleus matrix. Cytoplasm > cytoskeleton > spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Chromokinesin antibody
    • Chromokinesin-A antibody
    • Chromosome associated kinesin KIF4A antibody
    • Chromosome-associated kinesin KIF4A antibody
    • FLJ12530 antibody
    • FLJ12655 antibody
    • FLJ14204 antibody
    • FLJ20631 antibody
    • HSA271784 antibody
    • KIF4 antibody
    • KIF4 G1 antibody
    • KIF4A antibody
    • KIF4A_HUMAN antibody
    • KIF4G1 antibody
    • Kinesin family member 4A antibody
    see all

Anti-KIF4A antibody 图像

  • All lanes : Anti-KIF4A antibody (ab122227) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate
  • ab122227, at a 1/300 dilution, staining KIF4A in paraffin embedded Human bone marrow tissue by Immunohistochemistry.
  • Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli using ab122227.
    Recommended conditions:
    Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemical staining of Human bone marrow shows strong nuclear positivity in hematopoietic cells using ab122227

  • ab122227, at 4 µg/ml, staining KIF4A in Human cell line U-2 OS by Immunofluorescence. Antibody staining is shown in green.

Anti-KIF4A antibody (ab122227)参考文献

ab122227 has not yet been referenced specifically in any publications.

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