Anti-KIF3C抗体(ab89276)

概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to KIF3C
  • 经测试应用
    适用于: WB, IHC-FoFrmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein corresponding to amino acids 1-793 of Human KIF3C (NP_002245.4).

  • 阳性对照
    • HeLa cell lysate and KIF3C transfected 293T cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab89276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 89 kDa.
IHC-FoFr Use at an assay dependent concentration.

靶标

  • 功能
    Microtubule-based anterograde translocator for membranous organelles.
  • 序列相似性
    Belongs to the kinesin-like protein family. Kinesin II subfamily.
    Contains 1 kinesin-motor domain.
  • 细胞定位
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA4058 antibody
    • KIF3C antibody
    • KIF3C variant protein antibody
    • KIF3C_HUMAN antibody
    • Kinesin family member 3C antibody
    • Kinesin like protein KIF3C antibody
    • Kinesin-like protein KIF3C antibody
    • mKIAA4058 antibody
    see all

Anti-KIF3C antibody 图像

  • Anti-KIF3C antibody (ab89276) at 1 µg/ml + HeLa cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 89 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-KIF3C antibody (ab89276) at 1 µg/ml

    Lane 1 : KIF3C transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 89 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

Anti-KIF3C antibody (ab89276)参考文献

ab89276 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH6.0
Sample
Mouse Tissue sections (Cerebellum)
Specification
Cerebellum
Permeabilization
Yes - 0.3% Triton X-100, 30min, R.T.
Fixative
Paraformaldehyde
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提交于 Aug 19 2014

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