概述

  • 产品名称Anti-KIF3A抗体
    参阅全部 KIF3A 一抗
  • 描述
    兔多克隆抗体to KIF3A
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids 682-701 of Mouse KIF3A. This sequence is identical in human.

  • 阳性对照
    • Rat brain homogenate, PC12 cell line.
  • 常规说明Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

性能

应用

Our Abpromise guarantee covers the use of ab11259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 85 kDa. This concentration is determined by blotting using a rat brain homogenate and a whole cell extract of rat pheochromocytoma PC12 cell line. Staining of KIF3A is specifically inhibited by the KIF3A immunizing peptide.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 20421870

靶标

  • 功能Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro.
  • 序列相似性Belongs to the kinesin-like protein family. Kinesin II subfamily.
    Contains 1 kinesin-motor domain.
  • 细胞定位Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIF 3 antibody
    • KIF 3A antibody
    • KIF3 antibody
    • Kif3a antibody
    • KIF3A_HUMAN antibody
    • Kifl antibody
    • Kinesin family member 3A antibody
    • Kinesin family protein 3 antibody
    • Kinesin family protein 3A antibody
    • Kinesin like protein KIF 3A antibody
    • Kinesin like protein KIF3A antibody
    • Kinesin-like protein KIF3A antibody
    • Kns3 antibody
    • Microtubule plus end directed kinesin motor 3A antibody
    • Microtubule plus end-directed kinesin motor 3A antibody
    see all

Anti-KIF3A antibody 图像


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 85 kDa


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

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  • Predicted band size : 85 kDa

    This image is courtesy of an anonymous Abreview

    ab11259 (1/2000) detecting KIF3A in Human Hela Cells. As a control, cells were transfected with scramble siRNA (left) and KIF3A siRNA (right). Please refer to abreview for further experimental details.

    See Abreview

  • ab11259 staining KIF3A in murine primary hippocampal neurons by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed with methanol, blocked with 1% BSA for 1 hour at 22°C and then incubated with ab11259 at a 1/50 dilution for 2 hours at 22°C. The secondary used was a Cy3-conjugated donkey anti-rabbit polyclonal, used at a 1/500 dilution.

    See Abreview

Anti-KIF3A antibody (ab11259)参考文献

This product has been referenced in:
  • Hoang-Minh LB  et al. PCM1 Depletion Inhibits Glioblastoma Cell Ciliogenesis and Increases Cell Death and Sensitivity to Temozolomide. Transl Oncol 9:392-402 (2016). WB ; Human . Read more (PubMed: 27661404) »
  • Smith GA  et al. Progressive axonal transport and synaptic protein changes correlate with behavioral and neuropathological abnormalities in the heterozygous Q175 KI mouse model of Huntington's disease. Hum Mol Genet N/A:N/A (2014). Mouse . Read more (PubMed: 24728190) »

See all 15 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH-3T3 cells)
Permeabilization No
Specification NIH-3T3 cells
Blocking step 1% Donkey Serum + 1% BSA + 0.1% Triton X-100, in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Paraformaldehyde
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提交于 Nov 02 2015

Application Western blot
Sample Dog Cell lysate - whole cell (MDCK cells)
Loading amount 100 µg
Specification MDCK cells
Gel Running Conditions Reduced Denaturing (8% Tris-Glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 May 14 2013

Application Western blot
Sample African Green Monkey Cell lysate - whole cell (COS cells)
Loading amount 100 µg
Specification COS cells
Gel Running Conditions Reduced Denaturing (8% Tris-Glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 May 14 2013

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Application Western blot
Sample Cow Cell lysate - whole cell (MDBK cells)
Loading amount 100 µg
Specification MDBK cells
Gel Running Conditions Reduced Denaturing (8% Tris-Glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 May 13 2013

Application Western blot
Sample Syrian Hamster Cell lysate - whole cell (BHK cells)
Loading amount 100 µg
Specification BHK cells
Gel Running Conditions Reduced Denaturing (8% Tris-Glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Apr 16 2013

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Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 100 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (8% Tris-Glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Mar 28 2013

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Application Western blot
Sample Mouse Cell lysate - whole cell (NIH-3T3 cells)
Loading amount 100 µg
Specification NIH-3T3 cells
Gel Running Conditions Reduced Denaturing (8% Tris-Glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Mar 28 2013

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Application Western blot
Sample Rat Cell lysate - whole cell (NRK cells)
Loading amount 50 µg
Specification NRK cells
Gel Running Conditions Reduced Denaturing (8% Tris-Glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Mar 28 2013

Application Immunocytochemistry
Sample Mouse Cell (Primary hippocampal neurons)
Specification Primary hippocampal neurons
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
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提交于 Sep 09 2011

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Application Western blot
Sample Rat Tissue lysate - other (brain lysate differentially centrifuged, P3)
Loading amount 5 µg
Specification brain lysate differentially centrifuged, P3
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 May 24 2011

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"