概述

  • 产品名称Anti-Kif2a抗体
    参阅全部 Kif2a 一抗
  • 描述
    兔多克隆抗体to Kif2a
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Kif2a.

    (Peptide available as ab129223.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HEK293; HepG2; NIH3T3; MEF1; F9.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab86655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa).

靶标

  • 功能Plus end-directed microtubule-dependent motor required for normal brain development. May regulate microtubule dynamics during axonal growth. Required for normal progression through mitosis. Required for normal congress of chromosomes at the metaphase plate. Required for normal spindle dynamics during mitosis. Promotes spindle turnover. Implicated in formation of bipolar mitotic spindles. Has microtubule depolymerization activity.
  • 序列相似性Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
    Contains 1 kinesin-motor domain.
  • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Cytoplasm > cytoskeleton > spindle. Localized to the spindle microtubules and spindle poles from prophase to metaphase. Efficient targeting to spindle microtubules and spindle poles requires the kinase activity of PLK1. Recruited to mitotic spindles by interaction with PSRC1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDCBM3 antibody
    • HK2 antibody
    • KIF2 antibody
    • KIF2A antibody
    • KIF2A_HUMAN antibody
    • Kinesin 2 antibody
    • Kinesin heavy chain 2 antibody
    • Kinesin heavy chain member 2 antibody
    • Kinesin heavy chain member 2A antibody
    • Kinesin like protein KIF2A antibody
    • Kinesin-2 antibody
    • Kinesin-like protein KIF2A antibody
    • KNS2 antibody
    • M Kinesin antibody
    see all

Anti-Kif2a antibody 图像

  • All lanes : Anti-Kif2a antibody (ab86655) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 6 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 7 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 9 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 10 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 11 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 12 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 13 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 14 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

Anti-Kif2a antibody (ab86655)参考文献

ab86655 has not yet been referenced specifically in any publications.

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