概述

  • 产品名称
  • 描述
    兔多克隆抗体to Kif2a
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish, Orangutan
  • 免疫原

    Synthetic peptide: DLETQWGVGS SPQRDDLKLL CEQNEEEVSP QLFTFHEAVS QMVEMEEQVV , corresponding to C terminal amino acids 576 - 625 of Human Kif2a

  • 阳性对照
    • HepG2 cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab80492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Plus end-directed microtubule-dependent motor required for normal brain development. May regulate microtubule dynamics during axonal growth. Required for normal progression through mitosis. Required for normal congress of chromosomes at the metaphase plate. Required for normal spindle dynamics during mitosis. Promotes spindle turnover. Implicated in formation of bipolar mitotic spindles. Has microtubule depolymerization activity.
  • 序列相似性
    Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
    Contains 1 kinesin-motor domain.
  • 细胞定位
    Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Cytoplasm > cytoskeleton > spindle. Localized to the spindle microtubules and spindle poles from prophase to metaphase. Efficient targeting to spindle microtubules and spindle poles requires the kinase activity of PLK1. Recruited to mitotic spindles by interaction with PSRC1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDCBM3 antibody
    • HK2 antibody
    • KIF2 antibody
    • KIF2A antibody
    • KIF2A_HUMAN antibody
    • Kinesin 2 antibody
    • Kinesin heavy chain 2 antibody
    • Kinesin heavy chain member 2 antibody
    • Kinesin heavy chain member 2A antibody
    • Kinesin like protein KIF2A antibody
    • Kinesin-2 antibody
    • Kinesin-like protein KIF2A antibody
    • KNS2 antibody
    • M Kinesin antibody
    see all

Anti-Kif2a antibody 图像

  • Anti-Kif2a antibody (ab80492) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 - 1/100000

    Predicted band size : 80 kDa
    Observed band size : >90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

实验方案

Anti-Kif2a antibody (ab80492)参考文献

ab80492 has not yet been referenced specifically in any publications.

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